Subchronic exposure to arsenic induces apoptosis in the hippocampus of the mouse brains through the Bcl-2/Bax pathway

Journal of Occupational Health
Yachen WangFengyuan Piao

Abstract

The aim of this study was to identify whether arsenic (As) exposure could induce hippocampal neural apoptosis in vivo. Sixty-four mice were randomly divided into 4 groups of 16 each. Group 1 orally received drinking water alone as a control. Groups 2-4 were given arsenic trioxide (As2O3) orally at the doses of 1 ppm, 2 ppm and 4 ppm, respectively. All the treatments continued for 60 days. Morphological changes in the hippocampus were observed by HE staining. Apoptosis in the hippocampus was examined by TUNEL assay and transmission electron microscopy. The expression levels of Bcl-2 and Bax genes and their proteins in the hippocampus were determined by real-time PCR and Western blotting. The activity of caspase-3 was determined by spectrophotometry. Abnormal histopathological changes and apoptosis were found in the hippocampus of As-exposed mice. The expressions of the Bcl-2 gene and its protein in the hippocampus of As-exposed mice were significantly lower than those in the control group (p<0.05). However, the expressions of the Bax gene and its protein, and the expression ratio of Bax/Bcl-2 in the hippocampus were significantly higher in the groups exposed to As than in the control group (p<0.05). Moreover, the activity of cas...Continue Reading

References

Jul 24, 1998·American Journal of Epidemiology·M RahmanO Axelson
May 16, 2000·Environmental Health Perspectives·U K ChowdhuryD Chakraborti
Jul 12, 2001·Toxicology and Applied Pharmacology·U Namgung, Z Xia
Jul 27, 2001·Brain Research Bulletin·V M RodríguezM Giordano
Aug 6, 2002·Neuroscience Letters·Ali M SharifiMasoomeh Bakhshayesh
Oct 9, 2002·Toxicology Letters·Sukumar ChattopadhyayShyamal Das Gupta
Nov 28, 2002·The Journal of Biological Chemistry·Valery MikhailovPothana Saikumar
Dec 4, 2002·Neurotoxicology and Teratology·V M RodríguezM Giordano
Sep 10, 2003·Toxicology Letters·V M RodríguezM Giordano
Jun 7, 2006·Stress : the International Journal on the Biology of Stress·Jeansok J KimTherese A Kosten
May 5, 2009·Neurotoxicology and Teratology·Yanyan WangYufeng Zhao
May 23, 2009·Toxicology and Applied Pharmacology·Swaran J S FloraAshish Mehta
Jul 7, 2009·International Journal of Developmental Neuroscience : the Official Journal of the International Society for Developmental Neuroscience·Andreia Oliveira-da-SilvaYael Abreu-Villaça
Sep 10, 2009·Birth Defects Research. Part A, Clinical and Molecular Teratology·Saurabh SinghM Michele Pisano
Dec 27, 2011·Frontiers of Medicine·Kyoung-Woong KimSuthipong Sthiannopkao
Mar 13, 2012·Toxicological Sciences : an Official Journal of the Society of Toxicology·Liyun WangMakoto Shibutani
Sep 11, 2012·Reviews on Environmental Health·Maria ArgosJoseph H Graziano
Dec 12, 2012·Biometals : an International Journal on the Role of Metal Ions in Biology, Biochemistry, and Medicine·Xiaoxu WangFengyuan Piao
Mar 16, 2013·Annals of Hematology·Athena KritharisDaniel R Budman
May 14, 2014·International Journal of Oncology·Te-Chun HsiaJing-Gung Chung

❮ Previous
Next ❯

Citations

Jul 27, 2017·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·Quan MoYanchun Hu
Apr 1, 2020·Histochemistry and Cell Biology·Sherif Mohamed ZakiFatma M Ghoneim
Jan 18, 2018·International Journal of Molecular Sciences·Tianliang ZhangLi Yu
Feb 16, 2019·Journal of Cellular Physiology·Wan-Gen LiuRong Xiang
Nov 2, 2021·Frontiers in Behavioral Neuroscience·Tiantian ZhuPeng Li

❮ Previous
Next ❯

Related Concepts

Related Feeds

BCL-2 Family Proteins

BLC-2 family proteins are a group that share the same homologous BH domain. They play many different roles including pro-survival signals, mitochondria-mediated apoptosis and removal or damaged cells. They are often regulated by phosphorylation, affecting their catalytic activity. Here is the latest research on BCL-2 family proteins.

Apoptosis

Apoptosis is a specific process that leads to programmed cell death through the activation of an evolutionary conserved intracellular pathway leading to pathognomic cellular changes distinct from cellular necrosis

Related Papers

Experimental and Toxicologic Pathology : Official Journal of the Gesellschaft Für Toxikologische Pathologie
Zongyuan LiShuxian Qu
Food and Chemical Toxicology : an International Journal Published for the British Industrial Biological Research Association
Jin XuLi-hong Xu
© 2021 Meta ULC. All rights reserved