Abstract
The differing capacity of subclasses of IgG to bind to protein A and protein G was used in a sequential affinity purification procedure to examine immunoglobulin isotypes and subclasses in autoimmune disease. The utility of the procedure is that affinity-purified fractions containing particular isotypes and subclasses of immunoglobulin can be analyzed for their content of autoantibodies using standard techniques. For each of four autoimmune diseases studied, chronic active hepatitis, Sjogren's syndrome, primary biliary cirrhosis, and rheumatoid arthritis, there were characteristic protein elution profiles and the various disease-specific autoantibodies showed preferential distributions among the isotypes and subclasses. Moreover there was not an absolute correlation between an increased level of a particular subclass and the occurrence of antibodies of that subclass. The occurrence of highly disease-specific immunoglobulin subclass profiles suggests that the hypergammaglobulinemia associated with autoimmunity cannot be attributed entirely to polyclonal B-cell activation. Rather, there are disease-specific alterations in isotype subclass switching which may reflect different cytokine-dependent influences on autoimmune B cells an...Continue Reading
Citations
Dec 1, 1995·European Journal of Clinical Microbiology & Infectious Diseases : Official Publication of the European Society of Clinical Microbiology·M T CamachoA Tellez
Nov 4, 1995·Transfusion Science·E Rewald
Dec 31, 2003·Clinical Immunology : the Official Journal of the Clinical Immunology Society·Trinh T TranDavid S Pisetsky
Sep 25, 1998·The British Journal of Dermatology·K A WolpertS J Whittaker
Apr 12, 2001·The British Journal of Dermatology·E LaffitteL Borradori
Apr 1, 1995·Scandinavian Journal of Immunology·N KolbusM Mäder
Apr 1, 1995·Immunological Reviews·R L Coppel, M E Gershwin
Nov 25, 2000·Clinical and Experimental Immunology·H IllgesI Melchers
Aug 2, 2000·Proceedings of the National Academy of Sciences of the United States of America·N A Mitchison, L R Wedderburn
Dec 13, 2002·Oral Diseases·S SistigZ Kusić
Feb 20, 2010·Expert Opinion on Drug Metabolism & Toxicology·Dolores B Njoku
Sep 7, 2004·Clinical and Experimental Dermatology·I ChanJ A McGrath
Apr 12, 2005·European Journal of Oral Sciences·Nicolas DelaleuMarkus M Koller
Jul 24, 2010·Hepatology Research : the Official Journal of the Japan Society of Hepatology·Takashi HimotoTsutomu Masaki
Sep 1, 1996·Baillière's Clinical Gastroenterology·C Galperin, M E Gershwin
Jan 1, 1996·FEMS Immunology and Medical Microbiology·M B KalvenesG Haukenes
Sep 2, 2016·Frontiers in Microbiology·Marina I ArleevskayaAnatoly P Tsibulkin
Nov 30, 2011·Biologicals : Journal of the International Association of Biological Standardization·In Soo ShinSeung Hwa Hong
Feb 10, 2006·Clinical and Vaccine Immunology : CVI·Dolores B NjokuNoel R Rose