Subclinical Changes in Deceased Donor Kidney Proteomes Are Associated With 12-month Allograft Function Posttransplantation-A Preliminary Study

Transplantation
Maria KaisarRutger J Ploeg

Abstract

Cerebral injury during donation after brain death may induce systemic damage affecting long-term kidney function posttransplantation. Conventional evaluation of donor organ quality as a triage for transplantation is of limited utility. We compared donor kidneys yielding opposing extremes of the continuum of posttransplantation outcomes by several common kidney biopsy evaluation techniques, including Kidney Donor Profile Index and Remuzzi scoring, and analyzed tissue from a minimal sample cohort using label-free quantitation mass spectrometry. Further assessment of the proteomic results was performed by orthogonal quantitative comparisons of selected key proteins by immunoblotting. We show that common evaluation techniques of kidney biopsies were not predictive for posttransplantation outcomes. In contrast, despite the limited cohort size, the proteomic analysis was able to clearly differentiate between kidneys yielding extreme posttransplantation outcome differences. Pathway analysis of the proteomic data suggested that outcome-related variance in protein abundance associated with profibrotic, apoptosis, and antioxidant proteins. Immunoblotting confirmation further supported this observation. We present preliminary data indicat...Continue Reading

References

Apr 16, 2003·American Journal of Transplantation : Official Journal of the American Society of Transplantation and the American Society of Transplant Surgeons·Robert A MetzgerRobert M Merion
Feb 1, 2007·Transplant International : Official Journal of the European Society for Organ Transplantation·Cyril MoersRutger J Ploeg
Apr 27, 2011·Proteomics. Clinical Applications·Tara K SigdelMinnie M Sarwal
Jan 30, 2014·American Journal of Transplantation : Official Journal of the American Society of Transplantation and the American Society of Transplant Surgeons·B TanrioverM A Hardy
Feb 11, 2014·Clinical Transplantation·Chris J CallaghanJ Andrew Bradley
May 12, 2016·Kidney International·Tara K SigdelMinnie M Sarwal

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Citations

Oct 7, 2020·American Journal of Transplantation : Official Journal of the American Society of Transplantation and the American Society of Transplant Surgeons·Annemarie WeissenbacherBenedikt M Kessler
Dec 9, 2021·American Journal of Transplantation : Official Journal of the American Society of Transplantation and the American Society of Transplant Surgeons·Rebecca H VaughanMaria Kaisar

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Methods Mentioned

BETA
biopsy
biopsies
proteomic profiling
Proteomic

Software Mentioned

R
KDPI
PermutMatrix

Related Concepts

Related Feeds

Apoptosis

Apoptosis is a specific process that leads to programmed cell death through the activation of an evolutionary conserved intracellular pathway leading to pathognomic cellular changes distinct from cellular necrosis

Brain Injury & Trauma

brain injury after impact to the head is due to both immediate mechanical effects and delayed responses of neural tissues.