Subcloning the MAC-T bovine mammary epithelial cell line: morphology, growth properties, and cytogenetic analysis of clonal cells

Journal of Dairy Science
B ZavizionI Politis

Abstract

The objectives of the present study were 1) to determine the heterogeneity of the MAC-T cell line; 2) to examine whether homogeneous clones could be derived from MAC-T cells; and 3) to examine cell morphology, cytoskeletal characteristics, size, colony-forming ability, growth characteristics, beta-casein production, response to oxytocin, and cytogenetic properties of the clones. Three clonal cells, designated CU-1, CU-2, and CU-3, were derived from MAC-T cells. CU-1 and CU-2 cells were morphologically homogeneous. CU-3 cells were heterogeneous and contained two distinct subtypes. All clones contained cytokeratin 14 and 18. CU-2 and CU-3 cells were 30 and 18% larger, respectively, than CU-1 cells. CU-1 cells did not grow in serum-free medium. Doubling times for MAC-T, CU-2, and CU-3 were 46, 48, and 78 h, respectively, in serum-free medium. MAC-T cells and clones constitutively expressed beta-casein in culture ranging from .1 to .3 micrograms/ml per 24 h. Cytogenetic analyses revealed Robertsonian translocations and isochromosomes in the clonal lines. We conclude that parental MAC-T cells are heterogeneous in morphology, growth, and cytogenetic characteristics.

References

Dec 1, 1992·Journal of Dairy Science·B ZavizionR C Gorewit
Jan 1, 1992·Domestic Animal Endocrinology·I PolitisR Grosse
Mar 1, 1989·Journal of Reproduction and Fertility·I A Forsyth
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Citations

Nov 28, 2012·In Vitro Cellular & Developmental Biology. Animal·Ramakant KaushikManmohan Singh Chauhan
Nov 16, 2013·In Vitro Cellular & Developmental Biology. Animal·Magdalena Jedrzejczak, Iwona Szatkowska
Mar 1, 1996·In Vitro Cellular & Developmental Biology. Animal·B ZavizionI Politis
Apr 4, 2019·PloS One·Steven Grant Waldrop, Nammalwar Sriranganathan
Oct 19, 2011·Sexual Development : Genetics, Molecular Biology, Evolution, Endocrinology, Embryology, and Pathology of Sex Determination and Differentiation·P J DasT Raudsepp
Jan 25, 2012·In Vitro Cellular & Developmental Biology. Animal·Hui-Li TongDe-Yun Yin
Dec 15, 2015·American Journal of Physiology. Cell Physiology·Magdalena Arévalo TurrubiarteFrédéric Dessauge
Feb 2, 2010·Reproduction in Domestic Animals = Zuchthygiene·Y-M ZhengY Zhang
Jan 1, 1997·Biotechnology Advances·E MatitashviliB Zavizion

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