PMID: 6161370Nov 1, 1980Paper

Subcompartments of the G1 phase of cell cycle detected by flow cytometry

Proceedings of the National Academy of Sciences of the United States of America
Z DarzynkiewiczM R Melamed

Abstract

Cellular DNA and RNA were simultaneously quantitated in individual cells of cycling and quiescent populations by flow cytometry. Based on differences in RNA content, two distinct subcompartments, G1A and G1B, were recognized in the G1 phase of exponentially growing cells. After mitosis, cells reside in the low-RNA, G1A compartment from which they cannot enter the S phase directly. An increase in RNA above a critical level is required for G1 cells to be able to initiate DNA replication; G1 cells with RNA values above this level are classified as G1B. Cell transition from G1A to G1B and from G1B to S was analyzed in a stathmokinetic experiment on L1210 cells with a doubling time of 10 hr. The half-time of cell residence in the indeterminate state of G1 was found to be 1 hr, and the duration of the deterministic portion of G1 phase was 2 hr. The indeterminate state, although not identical with G1A, is most likely located in G1A. Cell quiescence induced by serum deprivation (3T3 cells) or by addition of n-butyrate (L1210) results in cell arrest at a state which, judged by RNA content, is similar to that of G1A of exponentially growing cells. The exit from this state, however, is much slower after stimulation of these blocked cells ...Continue Reading

References

Oct 1, 1975·Experimental Cell Research·Z DarzynkiewiczM R Melamed
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Jan 1, 1979·Proceedings of the National Academy of Sciences of the United States of America·Z DarzynkiewiczM R Melamed
Sep 1, 1979·Journal of Cellular Physiology·Z DarzynkiewiczM L Melamed
Aug 1, 1976·Proceedings of the National Academy of Sciences of the United States of America·Z DarzynkiewiczM R Melamed
Apr 1, 1973·Proceedings of the National Academy of Sciences of the United States of America·J A Smith, L Martin
Dec 1, 1974·Experimental Cell Research·L H Augenlicht, R Baserga
Jun 1, 1980·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·K D Bauer, L A Dethlefsen
Sep 1, 1980·Cytometry·Z DarzynkiewiczM R Melamed

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Citations

Jan 1, 1996·Cell Motility and the Cytoskeleton·J K Slack, P J Higgins
Jul 1, 1992·Biotechnology and Bioengineering·T I LinardosL A Behie
Feb 1, 1995·Journal of Cellular Physiology·K TakaseE W Gelfand
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Feb 15, 1988·Clinica Chimica Acta; International Journal of Clinical Chemistry·D H RyanP K Horan
Feb 15, 1990·Mechanisms of Ageing and Development·M YamamotoK Yamamoto
May 15, 1994·Journal of Biotechnology·K A HawboldtL A Behie
Jul 1, 1992·International Journal of Immunopharmacology·K MorikawaS Morikawa
Jan 1, 1994·Biochimie·R H CharollaisN S Thomas
Oct 10, 2002·Biochemical and Biophysical Research Communications·Tze HsiehJoseph Wu
Apr 30, 2003·Cellular Immunology·Timothy J HaleMonte V Hobbs
May 29, 1999·International Journal of Immunopharmacology·A Skowron-CendrzakJ Shani
Oct 24, 2002·Human Immunology·Urban J ScheuringArgyrios N Theofilopoulos
Oct 11, 2003·Biotechnology Advances·W YanpaisanP M Doran
Aug 1, 1985·Proceedings of the National Academy of Sciences of the United States of America·L KaczmarekR Baserga
Sep 1, 1985·Proceedings of the National Academy of Sciences of the United States of America·E SmelandR Ohlsson
Jun 1, 1986·Proceedings of the National Academy of Sciences of the United States of America·E A Clark, J A Ledbetter
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Jan 1, 1984·Cancer Investigation·F Traganos
Feb 18, 2010·Journal of Experimental Marine Biology and Ecology·Alison M RoarkChristiaan Leeuwenburgh

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