Subconjunctival dendrimer-drug therapy for the treatment of dry eye in a rabbit model of induced autoimmune dacryoadenitis

The Ocular Surface
Hui LinSamuel C Yiu

Abstract

To investigate the efficacy of a single subconjunctival injection of dendrimer-dexamethasone conjugate in a rabbit model of induced autoimmune dacryoadenitis (AID). Dendrimer biodistribution after subconjunctival injection in AID animals was evaluated using Cy5-labelled dendrimer (D-Cy5) and confocal microscopy. Diseased animals were treated with free dexamethasone (Free-Dex), dendrimer-dexamethasone (D-Dex), or saline via a single subconjunctival injection. The efficacy was evaluated using various clinical evaluations, such as Schirmer's test, tear breakup time (TBUT), and fluorescein and rose Bengal staining. Histopathology was evaluated by H&E staining and immunostaining. Levels of inflammatory cytokines and aquaporin proteins in the LGs were determined by real-time PCR. Subconjunctivally administered dendrimers selectively localized in the inflamed LGs, and were taken up by the infiltrating cells. At two weeks post single dose-treatment, the D-Dex group showed improved clinical evaluations. No significant changes were observed in other groups. H&E staining demonstrated less inflammatory cell infiltration and fewer atrophic acini in D-Dex group, compared to those treated with saline or Free-Dex. Immunohistochemistry demonstr...Continue Reading

Citations

Jul 16, 2020·Pharmaceutical Research·Anan ZhangXing Tang
Jun 23, 2020·Journal of Ophthalmology·Carlos Carpena-TorresGonzalo Carracedo
Sep 17, 2019·Translational Vision Science & Technology·Hui LinSamuel Yiu
Jul 1, 2020·Pharmaceutical Nanotechnology·Subramanian NatesanMoyad Shahwan
Jul 17, 2020·Biomedicine & Pharmacotherapy = Biomédecine & Pharmacothérapie·Flavia Rodrigues da SilvaPaula Santos Nunes

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