Subcritical water extraction of thyreostats from bovine muscle followed by liquid chromatography-tandem mass spectrometry

Food Additives & Contaminants. Part A, Chemistry, Analysis, Control, Exposure & Risk Assessment
Silvia RocchiAlessandra Gentili

Abstract

Thyreostats can be used fraudulently to promote a rapid increase in weight of breeding animals at low cost. Their severe toxicological effects impose the development of reliable analytical methods to be used in monitoring plans. This work describes an alternative approach to isolate residues of thiouracil, methyl-thiouracil, propyl-thiouracil, phenyl-thiouracil, tapazole and mercaptobenzimidazole from bovine muscle tissue. The developed procedure is based on the following three steps: i) matrix solid-phase dispersion with C18 for the preliminary sample preparation; ii) subcritical water extraction (SWE) at 160°C and 100 bar; iii) clean-up on an Oasis HLB cartridge. The quantitative determination was performed by LC-MS/MS in dual polarity ionization by using internal standardization. The SWE-LC-MS/MS method was validated according to the identification criteria of the Commission decision 2002/657/EC. The relative recoveries ranged from 72 to 97%; within-lab reproducibility was less than 18%. The decision limit and the detection capability of all analytes were below the recommended concentration, set at 10 µg kg-1, but the validation results demonstrated that this method could only be applied for screening of thiouracil and methy...Continue Reading

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Oct 4, 2016·Food Additives & Contaminants. Part A, Chemistry, Analysis, Control, Exposure & Risk Assessment·Alessandra GentiliLucia Mainero Rocca

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Citations

Jan 13, 2019·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Yahong HanXian Liu

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