Abstract
We wished to evaluate the effects of Pseudomonas aeruginosa (mannose-sensitive hemagglutination pilus strain, PA-MSHA) as an immunostimulating and anti-tumor agent for treatment of bladder cancer. Immunostimulating effects were assessed by the in vitro proliferation assay of murine splenic lymphocytes. Anti-tumor effects were studied in a subcutaneous tumor model established in female C57BL/6 mice using the MB49 bladder cell line. These mice received subcutaneous injections of normal saline (control group) or PA-MSHA (high, medium, or low dose, respectively, 1.6-2.0 × 10(9), 3.2- .0 × 10(8), 6.4-8.0 × 10(7) CFU/ml) twice a week for 3 weeks. Mice survival, tumor volume, vascular endothelial growth factor (VEGF) expression, microvessel density (MVD), serum levels of TNF-α and IFN-γ, and blood CD4(+) /CD8(+) counts were the study outcomes. We observed that PA-MSHA promoted the growth of splenic lymphocytes in vitro. In the murine tumor model, PA-MSHA prolonged mice survival and reduced tumor growth. Furthermore, VEGF and MVD were also diminished by PA-MSHA. Mice that received high and medium dose of PA-MSHA had significantly higher serum levels of IFN-γ and TNF-α (days 21 and 28), and higher levels of CD4(+) /CD8(+) cells (days 21...Continue Reading
References
Aug 1, 1976·The Journal of Urology·A MoralesA W Bruce
Dec 1, 1989·Immunological Reviews·P ScottA Sher
May 1, 1983·Infection and Immunity·J M PorwollR B Markham
Sep 1, 1993·The Journal of Urology·T L RatliffW J Catalona
Dec 15, 1993·Cancer·K FriedrichsW Jonat
Feb 1, 1997·The Journal of Urology·A R ZlottaK Huygen
Oct 26, 1999·European Journal of Cancer : Official Journal for European Organization for Research and Treatment of Cancer (EORTC) [and] European Association for Cancer Research (EACR)·Y H GanK Esuvaranathan
Jan 5, 2000·FEMS Immunology and Medical Microbiology·N LeeW J Park
Feb 7, 2001·Journal of Clinical Oncology : Official Journal of the American Society of Clinical Oncology·J P SteinD G Skinner
Mar 14, 2003·Current Opinion in Immunology·Thomas Blankenstein, Zhihai Qin
Jun 5, 2003·Nature Reviews. Cancer·Gabriele Bergers, Laura E Benjamin
Aug 6, 2003·The Journal of Experimental Medicine·Yunfei GaoZhinan Yin
Jan 8, 2005·Science·Rakesh K Jain
May 12, 2005·Angiogenesis·Chunhong GongBruce I Terman
Jul 18, 2006·Current Medicinal Chemistry·Girolamo RanieriDomenico Ribatti
Sep 27, 2008·Proceedings of the National Academy of Sciences of the United States of America·Chrysoula I LiakouPadmanee Sharma
Dec 5, 2008·Digestive Diseases and Sciences·Zhenyuan CaoHulun Li
Dec 6, 2008·Scandinavian Journal of Urology and Nephrology. Supplementum·D Maxwell Parkin
Jun 3, 2009·Journal of Clinical Oncology : Official Journal of the American Society of Clinical Oncology·Robert J MotzerRobert A Figlin
Oct 2, 2009·Journal of Nuclear Medicine : Official Publication, Society of Nuclear Medicine·Birgit PfostReingard Senekowitsch-Schmidtke
Jun 22, 2010·Neoplasia : an International Journal for Oncology Research·Carl-Jørgen ArumChun-Mei Zhao
Aug 17, 2011·Immunotherapy·Sergio A Quezada, Karl S Peggs
Jan 6, 2012·Gene Therapy·Z ZhaiR Rodriguez
Jun 9, 2012·Urology·Eddie Shu-yin ChanWarren D Heston
Jan 22, 2013·CA: a Cancer Journal for Clinicians·Rebecca SiegelAhmedin Jemal
Sep 24, 2013·International Immunopharmacology·Hechen ZhuTongwa Cao