Subcutaneous Injections of the Mannose-Sensitive Hemagglutination Pilus Strain of Pseudomonas aeruginosa Stimulate Host Immunity, Reduce Bladder Cancer Size and Improve Tumor Survival in Mice

Cell Biochemistry and Biophysics
Tao LiZhi-Ping Wang

Abstract

We wished to evaluate the effects of Pseudomonas aeruginosa (mannose-sensitive hemagglutination pilus strain, PA-MSHA) as an immunostimulating and anti-tumor agent for treatment of bladder cancer. Immunostimulating effects were assessed by the in vitro proliferation assay of murine splenic lymphocytes. Anti-tumor effects were studied in a subcutaneous tumor model established in female C57BL/6 mice using the MB49 bladder cell line. These mice received subcutaneous injections of normal saline (control group) or PA-MSHA (high, medium, or low dose, respectively, 1.6-2.0 × 10(9), 3.2- .0 × 10(8), 6.4-8.0 × 10(7) CFU/ml) twice a week for 3 weeks. Mice survival, tumor volume, vascular endothelial growth factor (VEGF) expression, microvessel density (MVD), serum levels of TNF-α and IFN-γ, and blood CD4(+) /CD8(+) counts were the study outcomes. We observed that PA-MSHA promoted the growth of splenic lymphocytes in vitro. In the murine tumor model, PA-MSHA prolonged mice survival and reduced tumor growth. Furthermore, VEGF and MVD were also diminished by PA-MSHA. Mice that received high and medium dose of PA-MSHA had significantly higher serum levels of IFN-γ and TNF-α (days 21 and 28), and higher levels of CD4(+) /CD8(+) cells (days 21...Continue Reading

References

May 1, 1983·Infection and Immunity·J M PorwollR B Markham
Sep 1, 1993·The Journal of Urology·T L RatliffW J Catalona
Dec 15, 1993·Cancer·K FriedrichsW Jonat
Oct 26, 1999·European Journal of Cancer : Official Journal for European Organization for Research and Treatment of Cancer (EORTC) [and] European Association for Cancer Research (EACR)·Y H GanK Esuvaranathan
Feb 7, 2001·Journal of Clinical Oncology : Official Journal of the American Society of Clinical Oncology·J P SteinD G Skinner
Mar 14, 2003·Current Opinion in Immunology·Thomas Blankenstein, Zhihai Qin
Jun 5, 2003·Nature Reviews. Cancer·Gabriele Bergers, Laura E Benjamin
Aug 6, 2003·The Journal of Experimental Medicine·Yunfei GaoZhinan Yin
Jul 18, 2006·Current Medicinal Chemistry·Girolamo RanieriDomenico Ribatti
Sep 27, 2008·Proceedings of the National Academy of Sciences of the United States of America·Chrysoula I LiakouPadmanee Sharma
Dec 6, 2008·Scandinavian Journal of Urology and Nephrology. Supplementum·D Maxwell Parkin
Jun 3, 2009·Journal of Clinical Oncology : Official Journal of the American Society of Clinical Oncology·Robert J MotzerRobert A Figlin
Oct 2, 2009·Journal of Nuclear Medicine : Official Publication, Society of Nuclear Medicine·Birgit PfostReingard Senekowitsch-Schmidtke
Aug 17, 2011·Immunotherapy·Sergio A Quezada, Karl S Peggs
Jan 22, 2013·CA: a Cancer Journal for Clinicians·Rebecca SiegelAhmedin Jemal

❮ Previous
Next ❯

Related Concepts

Related Feeds

Arterial-Venous in Development & Disease

Arterial-venous development may play a crucial role in cardiovascular diseases. Here is the latest research.