Suberin Regulates the Production of Cellulolytic Enzymes in Streptomyces scabiei, the Causal Agent of Potato Common Scab

Microbes and Environments
Rebeca Padilla-ReynaudCarole Beaulieu

Abstract

Suberin, a major constituent of the potato periderm, is known to promote the production of thaxtomins, the key virulence factors of the common scab-causing agent Streptomyces scabiei. In the present study, we speculated that suberin affected the production of glycosyl hydrolases, such as cellulases, by S. scabiei, and demonstrated that suberin promoted glycosyl hydrolase activity when added to cellulose-, xylan-, or lichenin-containing media. Furthermore, secretome analyses revealed that the addition of suberin to a cellulose-containing medium increased the production of glycosyl hydrolases. For example, the production of 13 out of the 14 cellulases produced by S. scabiei in cellulose-containing medium was stimulated by the presence of suberin. In most cases, the transcription of the corresponding cellulase-encoding genes was also markedly increased when the bacterium was grown in the presence of suberin and cellulose. The level of a subtilase-like protease inhibitor was markedly decreased by the presence of suberin. We proposed a model for the onset of S. scabiei virulence mechanisms by both cellulose and suberin, the main degradation product of cellulose that acts as an inducer of thaxtomin biosynthetic genes, and suberin pro...Continue Reading

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Citations

Dec 19, 2016·F1000Research·Keith F Chater
Nov 1, 2016·Microbes and Environments·Amadou SidibéCarole Beaulieu
Jun 23, 2018·Applied Microbiology and Biotechnology·Marie-Ève Lacombe-HarveyCarole Beaulieu
Jul 10, 2019·Annual Review of Phytopathology·Shree P ThapaJeff H Chang
Nov 12, 2019·Frontiers in Microbiology·Kate L HarveySteven P Djordjevic

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Methods Mentioned

BETA
acetylation
electrophoresis

Software Mentioned

SignalP
Phobius
SecretomeP
scaffold
Tatfind
Mascot

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