Subgroup J avian leukosis virus infection of chicken dendritic cells induces apoptosis via the aberrant expression of microRNAs

Scientific Reports
Di LiuMing Liao

Abstract

Subgroup J avian leukosis virus (ALV-J) is an oncogenic retrovirus that causes immunosuppression and enhances susceptibility to secondary infection. The innate immune system is the first line of defense in preventing bacterial and viral infections, and dendritic cells (DCs) play important roles in innate immunity. Because bone marrow is an organ that is susceptible to ALV-J, the virus may influence the generation of bone marrow-derived DCs. In this study, DCs cultured in vitro were used to investigate the effects of ALV infection. The results revealed that ALV-J could infect these cells during the early stages of differentiation, and infection of DCs with ALV-J resulted in apoptosis. miRNA sequencing data of uninfected and infected DCs revealed 122 differentially expressed miRNAs, with 115 demonstrating upregulation after ALV-J infection and the other 7 showing significant downregulation. The miRNAs that exhibited the highest levels of upregulation may suppress nutrient processing and metabolic function. These results indicated that ALV-J infection of chicken DCs could induce apoptosis via aberrant microRNA expression. These results provide a solid foundation for the further study of epigenetic influences on ALV-J-induced immun...Continue Reading

References

Jan 1, 1991·Annual Review of Immunology·R M Steinman
Apr 1, 1996·The Journal of Experimental Medicine·J M Austyn
Apr 1, 1998·Nature·J Banchereau, R M Steinman
Mar 29, 2006·Proceedings of the National Academy of Sciences of the United States of America·Ning Chai, Paul Bates
Mar 17, 2007·Avian Pathology : Journal of the W.V.P.A·Dana PrůkováJan Svoboda
May 18, 2007·Annual Review of Cell and Developmental Biology·Natascha Bushati, Stephen M Cohen
Nov 21, 2007·Proceedings of the National Academy of Sciences of the United States of America·Feng YangKaren L Beemon
Mar 6, 2009·Genome Biology·Ben LangmeadSteven L Salzberg
Nov 17, 2009·Nucleic Acids Research·Dennis A BensonEric W Sayers
Apr 9, 2010·The Journal of Immunology : Official Journal of the American Association of Immunologists·Jennifer JurkinHerbert Strobl
Nov 12, 2010·The Journal of Immunology : Official Journal of the American Association of Immunologists·Xingguang LiuXuetao Cao
Dec 30, 2011·Journal of Clinical Microbiology·Yulong GaoXiaomei Wang
Feb 7, 2012·Journal of Molecular Medicine : Official Organ of the Gesellschaft Deutscher Naturforscher Und Ärzte·Martin Busch, Alma Zernecke
Aug 1, 2012·Avian Pathology : Journal of the W.V.P.A·L N Payne, V Nair
Nov 20, 2012·Veterinary Microbiology·Qi WangXiaomei Wang
Feb 24, 2015·Nature Immunology·Manuel D Diaz-MuñozMartin Turner

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Citations

Jul 9, 2013·Trends in Microbiology·Jason Mercer, Urs F Greber
Jan 10, 2017·Frontiers in Immunology·Min Feng, Xiquan Zhang
Mar 17, 2020·The Journal of Poultry Science·Yang Wang
Jan 5, 2021·Frontiers in Veterinary Science·Silvia MirettiMario Baratta
Apr 27, 2021·Molecular Therapy. Nucleic Acids·Javid Sadri NahandHamed Mirzaei

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Datasets Mentioned

BETA
Z46390.1

Methods Mentioned

BETA
PCR
ELISA
flow cytometry
RNA-seq

Software Mentioned

RNAhybrid
R package
R
TargetScan
BLAST
GraphPad Prism
DEGseq

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