Sublingual immunization of trivalent human papillomavirus DNA vaccine in baculovirus nanovector for protection against vaginal challenge

PloS One
Hee-Jung LeeYoung Bong Kim

Abstract

Here, we report the immunogenicity of a sublingually delivered, trivalent human papillomavirus (HPV) DNA vaccine encapsidated in a human endogenous retrovirus (HERV) envelope-coated, nonreplicable, baculovirus nanovector. The HERV envelope-coated, nonreplicable, baculovirus-based DNA vaccine, encoding HPV16L1, -18L1 and -58L1 (AcHERV-triHPV), was constructed and sublingually administered to mice without adjuvant. Following sublingual (SL) administration, AcHERV-triHPV was absorbed and distributed throughout the body. At 15 minutes and 1 day post-dose, the distribution of AcHERV-triHPV to the lung was higher than that to other tissues. At 30 days post-dose, the levels of AcHERV-triHPV had diminished throughout the body. Six weeks after the first of three doses, 1×10(8) copies of SL AcHERV-triHPV induced HPV type-specific serum IgG and neutralizing antibodies to a degree comparable to that of IM immunization with 1×10(9) copies. AcHERV-triHPV induced HPV type-specific vaginal IgA titers in a dose-dependent manner. SL immunization with 1×10(10) copies of AcHERV-triHPV induced Th1 and Th2 cellular responses comparable to IM immunization with 1×10(9) copies. Molecular imaging revealed that SL AcHERV-triHPV in mice provided complete ...Continue Reading

References

Aug 5, 1997·Proceedings of the National Academy of Sciences of the United States of America·X MartinezC A Siegrist
Aug 10, 2000·Molecular Therapy : the Journal of the American Society of Gene Therapy·P A FieldsK A High
Dec 25, 2003·Journal of Virology·Christopher B BuckJohn T Schiller
Feb 15, 2005·Journal of Virology·Christopher B BuckJohn T Schiller
Feb 24, 2006·Nature Reviews. Immunology·Marian R Neutra, Pamela A Kozlowski
Jan 30, 2008·Proceedings of the National Academy of Sciences of the United States of America·Joo-Hye SongMi-Na Kweon
Nov 26, 2009·The Journal of Immunology : Official Journal of the American Association of Immunologists·Nicolas CuburuCecil Czerkinsky
Nov 17, 2011·Vaccine·Vincent PavotStéphane Paul
Sep 11, 2012·European Journal of Pharmaceutical Sciences : Official Journal of the European Federation for Pharmaceutical Sciences·Annika BordeAnette Larsson
Jul 26, 2013·Immune Network·Byoung-Shik ShimMan Ki Song
Apr 30, 2014·Clinical and Experimental Allergy : Journal of the British Society for Allergy and Clinical Immunology·Y NagaiS Sugawara
Jun 10, 2014·Journal of Controlled Release : Official Journal of the Controlled Release Society·Heleen KraanJean-Pierre Amorij
Jun 11, 2014·International Immunology·Tatsuhiko AzegamiHiroshi Kiyono
Oct 23, 2014·Scientific Reports·Alexander BelloGary P Kobinger

❮ Previous
Next ❯

Datasets Mentioned

BETA
NM014590

Methods Mentioned

BETA
enzyme-linked immunosorbent assay
ELISA

Software Mentioned

Living Image
SigmaStat
AID Elispot Reader

Related Concepts

Related Feeds

Avian Influenza: Innate Immune Adjuvant (ASM)

Adjuvants systems that are added to vaccines against avian influenza have be explored to enhance the innate immune system response against the virus. Here is the latest research on avian influenza and the innate immune adjuvant.

Cell-Type-Specific Viral Vectors

Viral vectors are used in biological research and therapy to deliver genetic material into cells. However, the efficiency of viral vectors varies depending on the cell type. Here is the latest research on cell-type-specific viral vectors.

Cell-Type Specific Viral Vectors

Viral vectors are used in biological research and therapy to deliver genetic material into cells. However, the efficiency of viral vectors varies depending on the cell type. Here is the latest research on cell-type-specific viral vectors.

Avian Influenza: Innate Immune Adjuvant

Adjuvants systems that are added to vaccines against avian influenza have be explored to enhance the innate immune system response against the virus. Here is the latest research on avian influenza and the innate immune adjuvant.

Cell-Type-Specific Viral Vectors (ASM)

Viral vectors are used in biological research and therapy to deliver genetic material into cells. However, the efficiency of viral vectors varies depending on the cell type. Here is the latest research on cell-type-specific viral vectors.