Sublytic complement C5b-9 complexes induce thrombospondin-1 production in rat glomerular mesangial cells via PI3-k/Akt: association with activation of latent transforming growth factor-beta1.

Clinical and Experimental Immunology
Ling-juan GaoJian-xia Tong

Abstract

Mesangial cell proliferation is a common cellular response to a variety of different types of glomerular injury. Complement C5b-9 is a prime candidate to mediate mesangial cell proliferation, especially sublytic C5b-9, which can induce the production of multiple inflammatory factors and cytokines. Transforming growth factor (TGF)-beta1 plays a major role in the accumulation of extracellular matrix (ECM), while thrombospondin (TSP)-1 has been identified as an activator of latent TGF-beta1 in an in vitro system. Using rat glomerular mesangial cells (GMCs) as a model system, we assessed the effect of sublytic C5b-9 on the expression of TSP-1 and TGF-beta1 and explored the relevant pathway of signal transduction. First, we ensured the concentrations of anti-Thy1 antibody and complement, which were regarded as a sublytic C5b-9 dose, and examined whether the sublytic C5b-9 induced expression of TSP-1 in rat GMCs which, in turn, activated latent TGF-beta1 by real-time polymerase chain reaction (PCR) and enzyme-linked immunosorbent assay (ELISA), respectively. Then, we investigated the role of the PI3-k/Akt pathway in sublytic C5b-9-induced TSP-1 production in rat GMCs by Western blot analysis. The addition of sublytic C5b-9 (5% anti-T...Continue Reading

References

May 1, 1988·The Journal of Clinical Investigation·J VaraniV M Dixit
Feb 21, 1997·Cell·T F FrankeL C Cantley
Jun 1, 1997·The International Journal of Biochemistry & Cell Biology·J C Adams
Apr 14, 1999·Proceedings of the National Academy of Sciences of the United States of America·L C Cantley, B G Neel
Aug 12, 2000·Immunology Today·M P WymannE Hirsch
Mar 7, 2001·Kidney International·W G CouserS J Shankland
Mar 29, 2001·Journal of the American Society of Nephrology : JASN·Natalia YevdokimovaRoger M Mason
Jun 26, 2001·Journal of the American Society of Nephrology : JASN·Duk-Hee KangRichard J Johnson
Aug 8, 2001·The Journal of Immunology : Official Journal of the American Association of Immunologists·L SoaneM L Shin
Aug 25, 2001·Experimental Biology and Medicine·I SargiannidouG P Tuszynski
Jun 1, 2002·Science·Lewis C Cantley
Aug 24, 2002·The Journal of Immunology : Official Journal of the American Association of Immunologists·Hongwei PengAndrey V Cybulsky
Oct 18, 2002·The Journal of Surgical Research·Sanjay KrishnaswamiGeorge P Tuszynski
May 23, 2003·Journal of the American Society of Nephrology : JASN·Haruko ShimamuraSei Sasaki
May 25, 2004·Seminars in Nephrology·Jonathan BarrattAlice C Smith
Sep 3, 2004·The Journal of Biological Chemistry·Geoffrey D Young, Joanne E Murphy-Ullrich

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Citations

Feb 7, 2016·Immunopharmacology and Immunotoxicology·Brijesh SutariyaMadhusudan N Saraf
Oct 29, 2013·Seminars in Nephrology·Tomoko TakanoAndrey V Cybulsky
May 10, 2011·Molecular Immunology·Trent M WoodruffFrancesco Tedesco
Oct 27, 2016·Immunological Reviews·Bryan Paul MorganDoryen Bubeck
Jun 26, 2017·Seminars in Cell & Developmental Biology·B Paul MorganDoryen Bubeck
Dec 18, 2013·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Jing ZhangYingwei Wang
Jul 9, 2013·Clinical Cancer Research : an Official Journal of the American Association for Cancer Research·Xiaohong WuPhilip O Livingston
Jun 23, 2021·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Longfei LiuYingwei Wang

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