PMID: 8611174Feb 1, 1996Paper

Submicromolar La3+ concentrations block the calcium release-activated channel, and impair CD69 and CD25 expression in CD3- or thapsigargin-activated Jurkat cells

The Biochemical Journal
C AusselJ P Breittmayer

Abstract

The calcium release-activated channel (CRAC) opened in Jurkat cells activated either with CD3 monoclonal antibody or the endoplasmic reticulum Ca2(+)-ATPase blocker, thapsigargin, is blocked by La3+ with an IC50 of 20 nM. Similarly, the entry of Mn2+, used as a surrogate for Ca2+, is also blocked by submicromolar La3+ concentrations. La3+ seems to play its role simply by plugging the CRAC because this ion does not penetrate the cells, as demonstrated by chelation experiments with EGTA. Blocking the Ca2+ influx in activated Jurkat cells results in a lack of expression of CD25, a chain of the interleukin-2 receptor and of CD69, a marker of T-cell activation. By contrast, the very early steps of the T-cell signalling pathway such as the release of Ca2+ from intracellular stores and the subsequent inhibition of phosphatidylserine synthesis are not affected by La3+.

Citations

Aug 4, 1998·The American Journal of Physiology·E R KokoskaT A Miller
Dec 31, 2005·Toxicological Sciences : an Official Journal of the Society of Toxicology·Kaori Mizota, Hiroshi Ueda
Jan 11, 2011·Journal of Neurochemistry·Cristian LazzariMaria Catia Sorgato
Jul 7, 2011·Molecular and Cellular Biology·Galina A GusarovaJacob I Sznajder
Sep 6, 2000·The Journal of Biological Chemistry·C R HalaszovichA Luckhoff
Nov 15, 2000·The Journal of Biological Chemistry·C Kunzelmann-MarcheM C Martínez
Mar 13, 2001·Annual Review of Immunology·R S Lewis
May 8, 2002·The Journal of Immunology : Official Journal of the American Association of Immunologists·Jan D LünemannFrauke Zipp
Oct 26, 2005·Journal of Leukocyte Biology·Gautham K Rao, Norbert E Kaminski
Aug 31, 2018·ELife·Xiaowei HouStephen Barstow Long
Mar 13, 2020·Frontiers in Plant Science·Quan-Fang ZhangNan Yao
May 12, 1998·The American Journal of Physiology·A KlishinL A Blatter
Aug 9, 2003·The Journal of Biological Chemistry·Xinpo JiangLyanne C Schlichter
Oct 10, 2002·American Journal of Physiology. Cell Physiology·Jaekyung Cecilia SongJeffrey B Matthews
Nov 26, 1999·Proceedings of the National Academy of Sciences of the United States of America·A Lepple-WienhuesF Lang
Sep 30, 2005·European Journal of Nuclear Medicine and Molecular Imaging·Stephanie GuillermetDaniel Fagret
Nov 28, 2012·Science·Xiaowei HouStephen B Long
Jul 23, 2014·Clinical and Experimental Allergy : Journal of the British Society for Allergy and Clinical Immunology·T NegoroY Nakano
Sep 17, 2009·Immunological Reviews·Murali Prakriya
Sep 25, 2015·Physiological Reviews·Murali Prakriya, Richard S Lewis
May 16, 2012·The Journal of Physiology·Beth A McNally, Murali Prakriya
Dec 6, 2012·Proceedings of the National Academy of Sciences of the United States of America·Gordon S DuncanTak W Mak
Aug 2, 2014·Molecular Cancer Therapeutics·Ute JungwirthWalter Berger
Mar 19, 2013·Biochimica Et Biophysica Acta·Stefania RomeoP Thomas Vernier
May 1, 2021·Journal of Colloid and Interface Science·Madeleine R FriesFrank Schreiber
Jan 13, 1998·Current Opinion in Biotechnology·M D Cahalan, K G Chandy
May 22, 2002·Cellular Signalling·Yolanda RománLuis M Botana
Oct 3, 2003·Biochemical and Biophysical Research Communications·P Thomas VernierMartin A Gundersen
Mar 17, 2004·Biochemical and Biophysical Research Communications·Vesa-Matti Loitto, Karl-Eric Magnusson
Aug 20, 2021·Toxicon : Official Journal of the International Society on Toxinology·Leila Staali, Didier A Colin

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