Submicrosecond surface-induced dissociation of peptide ions in a MALDI TOF MS

Analytical Chemistry
Chaminda M GamageV H Wysocki

Abstract

Surface-induced dissociation (SID) has been implemented in a matrix-assisted laser desorption/ionization time-of-flight mass spectrometer (MALDI TOF MS), allowing production of tandem mass spectrometric information for peptide ions (MALDI TOF SID TOF). The instrument retains the standard operational modes such as the reflectron monitoring of the MALDI-generated intact ions and postsource decay. We show through ion trajectory simulations and experimental results that implementing SID in a commercial MALDI TOF spectrometer is feasible and that the SID products in this instrument fall in an observation time frame that allows the specific detection of fast-fragmentation channels. The instrument design, pulse timing sequence, and high-voltage electronics together with SID spectra of MALDI-generated peptide ions are presented. Standard peptides such as YGGFLR, angiotensin III, fibrinopeptide A, and des-Arg1-bradykinin were dissociated by means of hyperthermal collisions with a gold surface coated with a self-assembled monolayer of 2-(perfluorodecyl)ethanethiol. With the extraction fields and the short observation times used, the spectra obtained show intense low-mass ion signals such as immonium, b2, b3, and y2 ions. TOF data analysi...Continue Reading

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Citations

Mar 17, 2006·Journal of the American Society for Mass Spectrometry·Facundo M FernandezJulia Laskin
Jan 15, 2008·Journal of the American Society for Mass Spectrometry·Vicki H WysockiRichard L Beardsley
Aug 24, 2012·Chemical Reviews·Jobin CyriacR G Cooks
Jun 22, 2016·Analytical Chemistry·Victor A MikhailovCarol V Robinson
Oct 8, 2008·Analytical Chemistry·Oh Kyu YoonRichard N Zare
Nov 16, 2005·Analytical Chemistry·Plamen A DemirevJeffrey S Lin
Nov 3, 2021·Chemical Reviews·Dalton T SnyderVicki H Wysocki

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