Subnuclear gene positioning through lamina association affects copper tolerance.

Nature Communications
Yuki SakamotoSachihiro Matsunaga

Abstract

The nuclear lamina plays an important role in the regulation of chromatin organization and gene positioning in animals. CROWDED NUCLEI (CRWN) is a strong candidate for the plant nuclear lamina protein in Arabidopsis thaliana but its biological function was largely unknown. Here, we show that CRWNs localize at the nuclear lamina and build the meshwork structure. Fluorescence in situ hybridization and RNA-seq analyses revealed that CRWNs regulate chromatin distribution and gene expression. More than 2000 differentially expressed genes were identified in the crwn1crwn4 double mutant. Copper-associated (CA) genes that form a gene cluster on chromosome 5 were among the downregulated genes in the double mutant exhibiting low tolerance to excess copper. Our analyses showed this low tolerance to copper was associated with the suppression of CA gene expression and that CRWN1 interacts with the CA gene locus, enabling the locus to localize at the nuclear lamina under excess copper conditions.

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Citations

Apr 6, 2021·Current Opinion in Plant Biology·Célia Baroux
May 25, 2021·Frontiers in Plant Science·Nan WangChang Liu
May 18, 2021·Frontiers in Plant Science·Jade BishopHans-Wilhelm Nützmann
Jun 29, 2021·Frontiers in Plant Science·Chieko GotoKentaro Tamura
Aug 17, 2021·Frontiers in Plant Science·Daniel LüdkeMarcel Wiermer

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Methods Mentioned

BETA
immunoprecipitation
super-resolution microscopies
co-immunoprecipitation
two-hybrid
Co-IP
Y2H
co-immunoprecipitation assay
RNA-seq
transgenic
ChIL

Software Mentioned

Bowtie
ImageJ
R package TCC
PANTHER
Huygens Professional
R

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