Suboptimal culture conditions induce more deviations in gene expression in male than female bovine blastocysts

BMC Genomics
Sonia HerasAnn Van Soom

Abstract

Since the development of in vitro embryo production in cattle, different supplements have been added to culture media to support embryo development, with serum being the most popular. However, the addition of serum during embryo culture can induce high birthweights and low viability in calves (Large Offspring Syndrome). Analysis of global gene expression in bovine embryos produced under different conditions can provide valuable information to optimize culture media for in vitro embryo production. We used RNA sequencing to examine the effect of in vitro embryo production, in either serum-containing or serum-free media, on the global gene expression pattern of individual bovine blastocysts. Compared to in vivo derived embryos, embryos produced in serum-containing medium had five times more differentially expressed genes than embryos produced in serum-free conditions (1109 vs. 207). Importantly, in vitro production in the presence of serum appeared to have a different impact on the embryos according to their sex, with male embryos having three times more genes differentially expressed than their female counterparts (1283 vs. 456). On the contrary, male and female embryos produced in serum-free conditions showed the same number (19...Continue Reading

References

Nov 26, 1998·Reviews of Reproduction·L E YoungI Wilmut
Dec 6, 2005·BMC Developmental Biology·Karen GoossensLuc J Peelman
Apr 6, 2006·Reproduction : the Official Journal of the Society for the Study of Fertility·D CorcoranP Lonergan
May 3, 2006·Journal of Lipid Research·G S TintShailendra B Patel
Jun 15, 2007·BMC Developmental Biology·Karen GoossensLuc J Peelman
Aug 31, 2007·Biostatistics·Mark D Robinson, Gordon K Smyth
Apr 25, 2009·Science·UNKNOWN Bovine Genome Sequencing and Analysis ConsortiumFeng-Qi Zhao
Oct 7, 2009·Human Molecular Genetics·Brenna A Market-VelkerMellissa R W Mann
Feb 6, 2010·Proceedings of the National Academy of Sciences of the United States of America·P Bermejo-AlvarezA Gutierrez-Adan
Mar 18, 2010·Animal Reproduction Science·Luiz Sergio Almeida CamargoJoão Henrique Moreira Viana
Dec 21, 2010·BMC Genomics·Wen Huang, Hasan Khatib
Apr 14, 2011·Reproduction : the Official Journal of the Society for the Study of Fertility·Isabelle CôtéClaude Robert
May 28, 2013·BMC Genomics·James L ChitwoodPablo J Ross
Mar 5, 2014·Proceedings of the National Academy of Sciences of the United States of America·Alexander GrafEckhard Wolf
Sep 5, 2014·BMC Genomics·Zongliang JiangXiuchun Cindy Tian
Sep 12, 2014·Reproduction, Fertility, and Development·Alfonso Gutierrez-AdanMellissa R W Mann

❮ Previous
Next ❯

Citations

Nov 1, 2016·Reproduction : the Official Journal of the Society for the Study of Fertility·Gael Cagnone, Marc-André Sirard
Apr 20, 2017·Frontiers in Genetics·Nicole GrossHasan Khatib
Mar 20, 2018·Animal : an International Journal of Animal Bioscience·M McGowanG Boe-Hansen
Feb 20, 2018·Molecular Reproduction and Development·Veronique Duranthon, Pascale Chavatte-Palmer
Nov 24, 2017·Reproduction, Fertility, and Development·Meriem HamdiDimitrios Rizos
Feb 6, 2019·Molecular Reproduction and Development·Priscila Ramos-IbeasAlfonso Gutiérrez-Adán
Mar 31, 2019·Epigenetics : Official Journal of the DNA Methylation Society·Anelise Dos Santos MendonçaMaurício Machaim Franco
Apr 11, 2019·Journal of Animal Science·Alan D EalySarah R McCoski
Feb 19, 2020·Annual Review of Animal Biosciences·Peter J Hansen
Oct 1, 2020·International Journal of Molecular Sciences·Ximo Garcia-DominguezFrancisco Marco-Jiménez
Sep 24, 2019·Molecular Reproduction and Development·Mohamed O TaqiDawit Tesfaye
Sep 4, 2019·Molecular Reproduction and Development·Caroline G LucasTiago Collares
Jan 5, 2021·Frontiers in Cell and Developmental Biology·Priscila Ramos-IbeasEnrique Gómez
Apr 3, 2020·Theriogenology·Qaisar ShahzadYangqing Lu
Jun 16, 2021·In Vitro Cellular & Developmental Biology. Animal·Ligiane Oliveira LemeMargot Alves Nunes Dode
Jul 3, 2021·International Journal of Molecular Sciences·Sebastian CanovasPilar Coy
Aug 31, 2021·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·María B RabaglinoHaja N Kadarmideen

❮ Previous
Next ❯

Datasets Mentioned

BETA
GSE74675

Methods Mentioned

BETA
RNAseq
PCA
RNA-seq
PCR
RNA
Chip

Software Mentioned

seq Analysis Tool
RNA
Cytoscape
geNorm
EdgeR
CLC Genomics Workbench
EdgeR Bioconductor
DGE
R
CLC

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.