Suboptimal SARS-CoV-2-specific CD8+ T cell response associated with the prominent HLA-A*02:01 phenotype

Proceedings of the National Academy of Sciences of the United States of America
Jennifer R HabelKatherine Kedzierska

Abstract

An improved understanding of human T cell-mediated immunity in COVID-19 is important for optimizing therapeutic and vaccine strategies. Experience with influenza shows that infection primes CD8+ T cell memory to peptides presented by common HLA types like HLA-A2, which enhances recovery and diminishes clinical severity upon reinfection. Stimulating peripheral blood mononuclear cells from COVID-19 convalescent patients with overlapping peptides from severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) led to the clonal expansion of SARS-CoV-2-specific CD8+ and CD4+ T cells in vitro, with CD4+ T cells being robust. We identified two HLA-A*02:01-restricted SARS-CoV-2-specfic CD8+ T cell epitopes, A2/S269-277 and A2/Orf1ab3183-3191 Using peptide-HLA tetramer enrichment, direct ex vivo assessment of A2/S269+CD8+ and A2/Orf1ab3183+CD8+ populations indicated that A2/S269+CD8+ T cells were detected at comparable frequencies (∼1.3 × 10-5) in acute and convalescent HLA-A*02:01+ patients. These frequencies were higher than those found in uninfected HLA-A*02:01+ donors (∼2.5 × 10-6), but low when compared to frequencies for influenza-specific (A2/M158) and Epstein-Barr virus (EBV)-specific (A2/BMLF1280) (∼1.38 × 10-4) populations. ...Continue Reading

References

Apr 2, 2016·Proceedings of the National Academy of Sciences of the United States of America·Sophie A ValkenburgKatherine Kedzierska
Apr 25, 2017·Clinical & Translational Immunology·Thi Ho NguyenKatherine Kedzierska
Feb 28, 2018·Nature Communications·Zhongfang WangKatherine Kedzierska
Feb 23, 2020·The Lancet Infectious Diseases·Ensheng DongLauren Gardner
Mar 30, 2020·Clinical Infectious Diseases : an Official Publication of the Infectious Diseases Society of America·Juanjuan ZhaoZheng Zhang
Apr 15, 2020·Viral Immunology·Katherine Kedzierska, Marios Koutsakos
Apr 22, 2020·Journal of Clinical Virology : the Official Publication of the Pan American Society for Clinical Virology·Yong HuYancheng Xu
May 1, 2020·Nature Medicine·Quan-Xin LongAi-Long Huang
May 14, 2020·Nature Medicine·Fatima AmanatFlorian Krammer
Jun 20, 2020·MMWR. Morbidity and Mortality Weekly Report·Erin K StokesKathleen E Fullerton
Jul 8, 2020·The Journal of Clinical Investigation·Yanqun WangJincun Zhao
Jul 28, 2020·Nature·Carolina LucasAkiko Iwasaki
Aug 14, 2020·Immunity·Runhong ZhouZhiwei Chen

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Citations

Dec 19, 2020·Frontiers in Microbiology·Tanveer AhmadSyed Mansoor Ali
Jan 23, 2021·Science Immunology·Anthony KusnadiChristian H Ottensmeier
Feb 2, 2021·IScience·Christopher SzetoStephanie Gras
Feb 10, 2021·Infectious Disease Reports·Andrew R McGillShyam S Mohapatra
Dec 17, 2020·Journal of Cellular and Molecular Medicine·Ziwei ChenYurong Tan
Jan 25, 2021·The Journal of Allergy and Clinical Immunology. in Practice·Mark Ballow, Christopher L Haga
Jan 8, 2021·Frontiers in Immunology·Anthony J Leonardi, Rui B Proenca
Jan 23, 2021·Advanced Drug Delivery Reviews·Saborni ChakrabortyTaia T Wang
Feb 21, 2021·Nature Communications·Adam K WheatleyStephen J Kent
Feb 11, 2021·Cell Reports Medicine·Marios KoutsakosKatherine Kedzierska
Mar 7, 2021·International Journal of Molecular Sciences·Angela DziedzicMichal Bijak
Apr 4, 2021·International Journal of Molecular Sciences·Fanny PojeroAnna Aiello
Apr 22, 2021·The Journal of Experimental Medicine·Jacques F Miller
Jun 16, 2021·Frontiers in Immunology·Alexander A LehmannPaul V Lehmann
Jun 22, 2021·EMBO Molecular Medicine·Simon HirschbergerSimone Kreth
Jun 26, 2021·Cell·Shira Weingarten-GabbayPardis C Sabeti
Jul 9, 2021·Cell Host & Microbe·Alba GrifoniAlessandro Sette
Jul 31, 2021·Frontiers in Immunology·Eleonora GalleraniFrancesco Nicoli
Aug 13, 2021·Frontiers in Immunology·Johannes ScheteligAlexander H Schmidt
Aug 21, 2021·Cellular & Molecular Immunology·Min-Seok Rha, Eui-Cheol Shin
Aug 20, 2021·Science Advances·Nader YatimCeleste Lebbé
Aug 15, 2021·The Journal of Immunology : Official Journal of the American Association of Immunologists·Tongcui MaNadia R Roan
Aug 31, 2021·Heliyon·I KaidashevD Maryniak
Sep 28, 2021·Frontiers in Immunology·Jiram Torres-RuizDiana Gómez-Martín

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Methods Mentioned

BETA
ELISA
fluorescence-activated cell sorter
FACS

Software Mentioned

NetMHCpan
NetCTLpan

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