PMID: 11927670Apr 3, 2002Paper

Subplasmalemmal endoplasmic reticulum controls K(Ca) channel activity upon stimulation with a moderate histamine concentration in a human umbilical vein endothelial cell line

The Journal of Physiology
M FriedenWolfgang F Graier

Abstract

This study was designed to elucidate the role of the subplasmalemmal endoplasmic reticulum (sER) in autacoid-induced stimulation of Ca(2+)-dependent K(+) channels in the umbilical vein endothelial cell-derived cell line EA.hy926. Cells were transfected with the Ca(2+) probe cameleon targeted to the ER for visualization of the ER network. A patch pipette was then placed close to or far (> 5 microm away) from the sER, single channel recordings (patch clamp technique) were monitored simultaneously with measurements of either ER Ca(2+) concentration (using the Ca(2+) probe Cam4-ER) or cytosolic free Ca(2+) concentration ([Ca(2+)](i); using fura-2) using a deconvolution imaging device. A voltage-dependent, large conductance Ca(2+)-dependent K(+) channel (BK(Ca); single channel conductance (gamma), 250 pS) was found. At membrane potentials of +40 and -40 mV, the EC(50) for Ca(2+) was 2.7 and 49.7 microM, respectively. In the vicinity of the sER, the BK(Ca) channel activity induced by 10 microM histamine was 32 times higher (open probability (P(o)) = 0.083 +/- 0.026) than in areas away from the sER (P(o) = 0.0026 +/- 0.002). However, at supramaximal histamine stimulation (100 microM), BK(Ca) channel activation was similar in patches i...Continue Reading

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Citations

Jun 15, 2010·The Journal of Membrane Biology·Joanne E MillershipJason I E Bruce
Oct 3, 2007·Pflügers Archiv : European journal of physiology·Magdalena M SzewczykAshok K Grover
Jan 22, 2003·Journal of Neuroscience Methods·Natasha Solovyova, Alexei Verkhratsky
Nov 2, 2002·British Journal of Pharmacology·Baskaran ThyagarajanKlaus Groschner
Jul 9, 2002·Clinical and Experimental Pharmacology & Physiology·Michaela M SpitalerWolfgang F Graier
Apr 30, 2003·Antioxidants & Redox Signaling·Victor E EsenabhaluWolfgang F Graier
Aug 21, 2012·World Journal of Biological Chemistry·Francesco MocciaFranco Tanzi
May 23, 2007·Proceedings of the National Academy of Sciences of the United States of America·Benjamin E SteinbergSergio Grinstein
Aug 19, 2011·Biochimie·Yves GouriouUmberto De Marchi
Jul 9, 2011·Biology of the Cell·Wei-Wei ShenNicolas Demaurex
May 18, 2010·Vascular Pharmacology·Alexander VangGaurav Choudhary
Dec 3, 2003·British Journal of Pharmacology·Cristina ZorattiWolfgang F Graier
Feb 7, 2006·Microcirculation : the Official Journal of the Microcirculatory Society, Inc·Iain N McSherryKim A Dora
Dec 22, 2005·The Journal of Biological Chemistry·Karin OsibowWolfgang F Graier
Feb 10, 2006·The Biochemical Journal·Karin OsibowWolfgang F Graier
Jan 22, 2005·The Journal of Biological Chemistry·Rolland MalliWolfgang F Graier
Jul 11, 2018·Cell Death & Disease·Andreas YiallourisAnastasis Stephanou
Jan 17, 2003·The Journal of Biological Chemistry·Roland MalliWolfgang F Graier

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