Subpopulations of human granulosa-luteal cells obtained during early timed and during normally timed follicular aspiration in in-vitro fertilization-embryo transfer cycles

Fertility and Sterility
K GersakH Meden-Vrtovec

Abstract

To find the differences between human granulosa-luteal cells obtained during early timed follicular aspiration to prevent severe ovarian hyperstimulation syndrome (OHSS) and during normally timed follicular aspiration. Retrospective analysis of clinical laboratory data. In vitro fertilization unit, University Department of Obstetrics and Gynecology, Ljubljana, Slovenia. Twenty women undergoing IVF-ET at high risk for OHSS. Cells were obtained from the follicles of women who were stimulated with hMG and hCG during an early timed follicular aspiration of one ovary, 10-12 hours after hCG, and during a normally timed follicular aspiration of the contralateral ovary, 32-36 hours after hCG administration. Subpopulations of granulosa-luteal cells were observed by computerized image analysis in which hCG was localized using immunoperoxidase staining. Early timed follicular aspirates contained no oocytes and only a scant number of granulosa cells. Granulosa-luteal cells were smaller than those from normally timed follicular aspirates. We identified three subpopulations in early timed follicular aspirates: nonluteinized, small luteinized, and medium luteinized cells. In normally timed follicular aspirates, four subpopulations were identi...Continue Reading

References

Oct 1, 1992·Journal of Assisted Reproduction and Genetics·P A Bergh, D Navot
Nov 1, 1988·Biology of Reproduction·J L Cavender, W J Murdoch
Jul 1, 1987·The Journal of Clinical Endocrinology and Metabolism·J E SealeyJ H Laragh
Mar 8, 1985·Science·J G Cannon, C A Dinarello
Jan 1, 1995·Human Reproduction·R OrvietoZ Ben-Rafael
Jun 30, 1994·European Journal of Obstetrics, Gynecology, and Reproductive Biology·M Tsirigotis, I Craft
Apr 1, 1996·Journal of Assisted Reproduction and Genetics·T Tomazevic, H Meden-Vrtovec

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