PMID: 9178575Mar 1, 1997Paper

Subpopulations of stromal cells from long-term human bone marrow cultures: ontogeny of progenitor cells and expression of growth hormone receptors

Anatomia, histologia, embryologia
D T LincolnM J Waters

Abstract

Long-term culture of bone marrow derived stromal colony forming cells (S-CFC) in matrix and nutrient defined agar medium resulted in stromal cell colonies that pass sequentially through three distinct morphological stages: firstly, aggregated loose syncytium of round to avoid cells (stage I), a second developmental stage of large branching colonies in which the cells become enlarged, elongated with cytoplasmic projections forming a loosely anastomized network with adjacent cells (stage II), and finally cells become dissociated, loosing their long, thin cytoplasmic filaments and breaking their contacts with one another, but remain large and retain a bi-polar nature (stage III). Cells were also grown in liquid medium in a culture microenvironment closely resembling conditions of haemopoiesis in vitro. Using a panel of well defined monoclonal antibodies reactive against the rat, rabbit and human growth hormone receptors, this study found immunochemical evidence of the presence and localization of binding sites of growth hormone (GH) in the cell membrane and extra-nuclear Golgi area of long-term bone marrow derived human stromal cells in liquid and semi-solid nutrient agar mediums. GH-receptor immunoreactivity was present in small ...Continue Reading

References

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Citations

Dec 4, 2014·Growth Hormone & IGF Research : Official Journal of the Growth Hormone Research Society and the International IGF Research Society·Jessie R WangStephen J Yarwood
Feb 17, 2007·General and Comparative Endocrinology·Steve HarveyEsmond J Sanders
Jun 16, 2005·Stem Cells·Mary B HanleyJoseph M McCune
Nov 2, 2005·Biochemical and Biophysical Research Communications·Simon M CoolMichael J Waters
Apr 6, 2001·The American Journal of Pathology·M Gebre-MedhinJ M Meis-Kindblom
May 23, 2008·Differentiation; Research in Biological Diversity·Roger S JacksonNeil A Bhowmick
Jan 2, 2008·Growth Hormone & IGF Research : Official Journal of the Growth Hormone Research Society and the International IGF Research Society·Jessica K DevinPampee P Young
Aug 13, 1999·The American Journal of Physiology·B B BrimhallM E Sunday

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