Subretinal Transplantation of Human Amniotic Epithelial Cells in the Treatment of Autoimmune Uveitis in Rats

Cell Transplantation
Jinying LiLuyang Yu

Abstract

As a featured ocular inflammatory disease, autoimmune uveitis is the major cause of blindness in the clinic. Although current immunosuppressive regimens can alleviate the progression of autoimmune uveitis, they have serious side effects. Therefore, an alternative therapeutic strategy is urgently required. The present study investigated the therapeutic efficacy of human amniotic epithelial cells (hAECs) on autoimmune uveitis in a rat model. Herein, experimental autoimmune uveitis (EAU) was induced in rats via a subcutaneous injection of interphotoreceptor retinoid-binding protein. EAU rats were treated with hAECs or the vehicle solution via a subretinal injection on day 0 and day 6 after immunization, and rats were sacrificed on day 12 and day 18 for further analysis. The pathological development of EAU was evaluated by slit lamp microscopy. Immune cell infiltration and retinal structure damage were examined by histological examination of hematoxylin and eosin (H&E) and immunofluorescence staining. T-cell subsets were detected by flow cytometry, and the levels of inflammatory cytokines were quantified by enzyme-linked immunosorbent assay (ELISA). hAEC treatment ameliorated the pathological progression of EAU and preserved the re...Continue Reading

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Citations

Jun 19, 2019·Inflammation Research : Official Journal of the European Histamine Research Society ... [et Al.]·Xuewei YinDadong Guo
Jan 23, 2021·International Journal of Molecular Sciences·Quan-Wen LiuHong-Bo Xin
Oct 23, 2020·International Journal of Molecular Sciences·Chen QiuJinying Li
Aug 17, 2021·Frontiers in Immunology·Dragana Odobasic, Stephen R Holdsworth
Oct 16, 2021·Frontiers in Cell and Developmental Biology·Jinying LiLuyang Yu

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Methods Mentioned

BETA
Cesarean section
enzyme-linked immunosorbent assay
flow cytometry
ELISA

Software Mentioned

GraphPad Prism

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