Subset of Cortical Layer 6b Neurons Selectively Innervates Higher Order Thalamic Nuclei in Mice

Cerebral Cortex
Anna Hoerder-SuabedissenZoltán Molnár

Abstract

The thalamus receives input from 3 distinct cortical layers, but input from only 2 of these has been well characterized. We therefore investigated whether the third input, derived from layer 6b, is more similar to the projections from layer 6a or layer 5. We studied the projections of a restricted population of deep layer 6 cells ("layer 6b cells") taking advantage of the transgenic mouse Tg(Drd1a-cre)FK164Gsat/Mmucd (Drd1a-Cre), that selectively expresses Cre-recombinase in a subpopulation of layer 6b neurons across the entire cortical mantle. At P8, 18% of layer 6b neurons are labeled with Drd1a-Cre::tdTomato in somatosensory cortex (SS), and some co-express known layer 6b markers. Using Cre-dependent viral tracing, we identified topographical projections to higher order thalamic nuclei. VGluT1+ synapses formed by labeled layer 6b projections were found in posterior thalamic nucleus (Po) but not in the (pre)thalamic reticular nucleus (TRN). The lack of TRN collaterals was confirmed with single-cell tracing from SS. Transmission electron microscopy comparison of terminal varicosities from layer 5 and layer 6b axons in Po showed that L6b varicosities are markedly smaller and simpler than the majority from L5. Our results sugges...Continue Reading

References

Sep 1, 1996·Journal of Neurophysiology·S M Sherman, R W Guillery
Dec 22, 1998·Brain Research. Brain Research Reviews·M DeschênesZ W Zhang
Nov 11, 1999·Proceedings of the National Academy of Sciences of the United States of America·E S LeinC J Shatz
Jul 25, 2000·The Journal of Comparative Neurology·P VeinanteM Deschênes
Oct 4, 2000·Visual Neuroscience·A W PrzybyszewskiD A Pollen
Oct 12, 2000·The Journal of Comparative Neurology·R T RobertsonJ Yu
Feb 17, 2001·Brain Research Bulletin·E M Rouiller, E Welker
Oct 18, 2002·The European Journal of Neuroscience·P BarthóL Acsády
Mar 11, 2003·Philosophical Transactions of the Royal Society of London. Series B, Biological Sciences·Edward G Jones
Jul 26, 2003·Science·Patrick O KanoldCarla J Shatz
May 14, 2004·Journal of Neurophysiology·Iva Reichova, S Murray Sherman
Jul 30, 2004·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Laurence BayerMichel Mühlethaler
Jan 24, 2007·Proceedings of the National Academy of Sciences of the United States of America·Ian M AndolinaAdam M Sillito
Jan 24, 2007·The European Journal of Neuroscience·Erin C JacobsAnthony T Campagnoni
Sep 15, 2007·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Shiaoching GongCharles R Gerfen
Sep 26, 2008·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Alexander GrohThomas Kuner
Nov 15, 2008·Cerebral Cortex·Anna Hoerder-SuabedissenZoltán Molnár
May 19, 2009·Cerebral Cortex·Ying-Wan Lam, S Murray Sherman
Jun 6, 2009·Brain Research·Leeyup ChungCharles L Cox
Jul 30, 2011·The Journal of Comparative Neurology·Anna Hoerder-Suabedissen, Zoltán Molnár
Aug 25, 2011·Cerebral Cortex·Franziska M OeschgerZoltán Molnár
Aug 27, 2011·Neuron·T Grant BelgardChris P Ponting
Jan 13, 2012·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Else A TolnerPatrick O Kanold
Jan 17, 2012·Nature Methods·Timothy RaganPavel Osten
Feb 4, 2012·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Sarada ViswanathanPatrick O Kanold
Mar 1, 2012·Nature·Shawn R OlsenMassimo Scanziani
May 26, 2012·Cerebral Cortex·Anna Hoerder-Suabedissen, Zoltán Molnár
Feb 13, 2013·Proceedings of the National Academy of Sciences of the United States of America·Anna Hoerder-SuabedissenZoltán Molnár
Jul 5, 2013·Cerebral Cortex·Alexander GrohLászló Acsády
Apr 30, 2014·Proceedings of the National Academy of Sciences of the United States of America·María PedrazaJuan A De Carlos
Jun 27, 2014·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Xiangying MengPatrick O Kanold

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Citations

May 29, 2018·The European Journal of Neuroscience·Zoltán Molnár
Dec 7, 2018·The Journal of Comparative Neurology·Jacqueline BoonAnna Hoerder-Suabedissen
Jun 8, 2019·Journal of Anatomy·Goran Sedmak, Miloš Judaš
May 29, 2020·Proceedings of the National Academy of Sciences of the United States of America·Megan A KirchgessnerEdward M Callaway
Jun 9, 2020·The Journal of Comparative Neurology·Joel D HahnHong-Wei Dong
Jul 1, 2020·The Journal of Physiology·Josephine AnsorgePatrik Krieger
Sep 7, 2018·Brain Structure & Function·Minzi Chang, Hideki Derek Kawai
Nov 30, 2018·Frontiers in Neuroanatomy·Heiko J LuhmannWerner Kilb
May 28, 2019·Frontiers in Neuroanatomy·Sheena Yin Xin TiongMakoto Sato
Apr 9, 2020·Frontiers in Neuroanatomy·Chiaki Ohtaka-Maruyama
Apr 9, 2020·The Journal of Comparative Neurology·Hannah BruguierZoltán Molnár
May 28, 2020·Frontiers in Systems Neuroscience·Malinda L S TantirigamaRobert N S Sachdev
Nov 22, 2018·Frontiers in Neuroanatomy·Catherine MaclachlanGraham Knott
Mar 4, 2019·The Cerebellum·Christophe HabasPierre Cabaraux
Sep 5, 2019·The Journal of Comparative Neurology·Elena BorraKathleen S Rockland
May 2, 2019·Frontiers in Neuroanatomy·Patrick O KanoldXiangying Meng
Nov 24, 2020·The Journal of Comparative Neurology·Bruno Oliveira Ferreira de SouzaDenis Boire
Mar 8, 2021·Neuroscience and Biobehavioral Reviews·Jean-Christophe Cassel, Anne Pereira de Vasconcelos
Mar 27, 2021·Neuroscience and Biobehavioral Reviews·Christian O'ReillySean L Hill
Apr 28, 2021·Progress in Neurobiology·Navneet A Vasistha, Konstantin Khodosevich
Apr 29, 2021·Journal of the Association for Research in Otolaryngology : JARO·Minzi Chang, Patrick O Kanold
May 8, 2021·Nature Reviews. Neuroscience·Gordon M G Shepherd, Naoki Yamawaki
Apr 29, 2021·The Journal of Comparative Neurology·Courtney Michelle WhildenSolange Pezon Brown
May 21, 2021·Proceedings of the National Academy of Sciences of the United States of America·Daniel Z DoyleKenneth Y Kwan
Oct 7, 2021·Current Biology : CB·Megan A KirchgessnerEdward M Callaway
Dec 21, 2021·The European Journal of Neuroscience·Tobias Maximilian Breuer, Patrik Krieger

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Methods Mentioned

BETA
transgenic
transmission electron microscopy
Fluorescence
imaging procedure

Software Mentioned

GraphPad Prism
Nis
Volocity
ImageJ
Elements®

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