Subsets of CD34+ cells and rapid hematopoietic recovery after peripheral-blood stem-cell transplantation

Journal of Clinical Oncology : Official Journal of the American Society of Clinical Oncology
M W DercksenC E van der Schoot

Abstract

To study whether there is a relationship between transplanted cell dose and rate of hematopoietic recovery after peripheral-blood stem-cell (PBSC) transplantation, and to obtain an indication whether specific subsets of CD34+ cell populations contribute to rapid recovery of neutrophils or platelets. Based on data from 59 patients, we calculated for each day after PBSC transplantation the dose of CD34+ cells that resulted in rapid recovery of either neutrophils or platelets in the majority (> 70%) of patients. Using dual-color flow cytometry, subsets of peripheral-blood CD34+ cells were quantified and the numbers of CD34+ cells belonging to each of the reinfused subsets correlated with hematopoietic recovery following high-dose chemotherapy. The calculated threshold values with a high probability of engraftment showed a steep dose-effect relationship between CD34+ cell dose and time to recovery of both neutrophils or platelets. Predominantly CD34+ cells with the phenotype of myeloid precursors were mobilized. A minority of CD34+ cells expressed the erythroid and megakaryocytic lineage-associated antigens and a low but distinct population of CD34+ cells expressed antigens associated with multipotent stem cells. Analysis showed th...Continue Reading

Citations

Mar 15, 2000·Journal of Clinical Oncology : Official Journal of the American Society of Clinical Oncology·S SienaC Carlo-Stella
Nov 22, 2005·The Korean Journal of Internal Medicine·Jin Seok AhnMyung-Ju Ahn
Feb 21, 2006·Journal of Interferon & Cytokine Research : the Official Journal of the International Society for Interferon and Cytokine Research·J CaseA Manoharan
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Jul 25, 2000·European Journal of Clinical Investigation·P M RoodC E van der Schoot
Apr 29, 1999·Transfusion·A G SmolowiczU Tidefelt

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