Substance P activation of bronchoalveolar macrophages from asthmatic patients and normal subjects.

Clinical and Experimental Allergy : Journal of the British Society for Allergy and Clinical Immunology
J L PujolF B Michel

Abstract

The role of substance P in the pathogenesis of asthma is unclear. Animal studies suggest that it may be important, whereas human studies do not confirm this. Alveolar macrophages can be recovered easily by bronchoalveolar lavage (BAL) and stimulated in vitro. To assess the role of substance P in humans, we tested its ability to stimulate alveolar macrophages from six normal subjects and seven asthmatic patients. BAL cells were separated by adherence and alveolar macrophages constituted 95% of the adherent cell population. Four concentrations of substance P were used (10(-7), 10(-6), 10(-5), 10(-4) M). To assess the non-specific activation of alveolar macrophages we used three concentrations of lipopolysaccharides (LPS) (5, 10, 20 micrograms/ml). The stimulation of alveolar macrophages was assessed by the release of thromboxane B2 by radioimmunoassay. This study indicates that alveolar macrophages are stimulated by LPS but are poorly activated or not at all by substance P.

References

Jul 29, 1975·European Journal of Clinical Investigation·F DrayJ Maclouf
Feb 1, 1986·Lancet·P J Barnes
Aug 1, 1987·The Journal of Allergy and Clinical Immunology·M CluzelP Godard
Oct 15, 1982·European Journal of Pharmacology·J W Growcott, A V Tarpey
Aug 1, 1982·The Journal of Allergy and Clinical Immunology·P GodardF B Michel

❮ Previous
Next ❯

Citations

Aug 1, 1991·Immunology and Cell Biology·A EglezosR D Helme
Apr 15, 1992·Annals of the New York Academy of Sciences·A WozniakG McLennan
Feb 26, 1998·American Journal of Respiratory Cell and Molecular Biology·G W HoyleM Friedman
Oct 12, 2010·Respiratory Research·Stanislas Grassin-DelylePhilippe Devillier
May 1, 1996·Immunological Investigations·A E El-ShazlyT Ishikawa
Oct 1, 1992·The Journal of Allergy and Clinical Immunology·K NieberG Kunkel

❮ Previous
Next ❯

Related Concepts

Related Feeds

Allergy and Asthma

Allergy and asthma are inflammatory disorders that are triggered by the activation of an allergen-specific regulatory t cell. These t cells become activated when allergens are recognized by allergen-presenting cells. Here is the latest research on allergy and asthma.

Asthma

This feed focuses in Asthma in which your airways narrow and swell. This can make breathing difficult and trigger coughing, wheezing and shortness of breath.