Substance P enhances the local activation of NK1 R-expressing c-kit+ cardiac progenitor cells in right atrium of ischemia/reperfusion-injured heart

BMC Molecular and Cell Biology
Yun-Mi JeongWeon Kim

Abstract

Localization of neurokinin 1 receptor (NK1R), the endogenous receptor for neuropeptide substance P (SP), has already been described for the right atrium (RA) of the heart. However, the biological role of SP/NK1R signal pathways in the RA remains unclear. Sprague-Dawley rats were randomly divided into 4 groups (n = 22 each); subjected to sham, ischemia/reperfusion-injury (I/R), I/R with 5 nmole/kg SP injection (SP + I/R), and SP + I/R with 1 mg/kg RP67580 injection (RP, a selective non-peptide tachykinin NK1R antagonist) (RP/SP + I/R). The left anterior descending coronary artery was occluded for 40 min followed by 1 day reperfusion with SP or SP + RP or without either. After 1 day, both atria and ventricles as well as the heart apexes were collected. SP promoted the expression of c-Kit, GATA4, Oct4, Nanog, and Sox2 in only the RA of the SP + I/R rats via NK1R activation. In agreement with these observations, NK1R-expressing c-Kit+ Nkx2.5+GATA4+ cardiac progenitor cells (CPCs) in the ex vivo RA explant outgrowth assay markedly migrated out from RA1 day SP + I/R approximately 2-fold increase more than RA1 day I/R. Treatment of SP promoted proliferation, migration, cardiosphere formation, and potential to differentiate into cardio...Continue Reading

References

Sep 12, 2000·Journal of Hematotherapy & Stem Cell Research·Y LiW Ho
Jul 8, 2009·Stem Cells and Development·Shinka MiyamotoHiromi Kurosawa
Dec 20, 2011·Bioconjugate Chemistry·Shahir S RizkAnthony A Kossiakoff
Mar 31, 2012·ISRN Cardiology·Mani ArsalanJens Garbade
Jan 16, 2015·Radiographics : a Review Publication of the Radiological Society of North America, Inc·Sachin B MalikJoe Y Hsu
Apr 30, 2015·The Spine Journal : Official Journal of the North American Spine Society·Kyoung-Tae KimSeung-Won Park
Apr 30, 2015·The Journal of Nutrition, Health & Aging·Y ZhouK Tang
Mar 5, 2016·International Journal of Molecular Medicine·Maria Jose Dubon, Ki-Sook Park
Apr 21, 2016·Development·Maria Paola SantiniJason C Kovacic
May 14, 2017·Journal of Cellular Biochemistry·Miguel MuñozAntonio Ayala
Jun 29, 2017·Pharmacological Research : the Official Journal of the Italian Pharmacological Society·Iolanda AquilaDaniele Torella
Dec 15, 2017·Journal of Stem Cell Research & Therapy·Chuan LiAtsushi Amano
Jun 10, 2018·International Journal of Cardiology·Doo Sun SimMyung Ho Jeong
Jul 31, 2018·Frontiers in Physiology·Magdalena Chottova DvorakovaJana Slavikova
Sep 5, 2018·Basic Research in Cardiology·Julia KrauseJustus Stenzig
Nov 13, 2018·Stem Cell Reports·Marta LemmeThomas Eschenhagen
Jan 8, 2019·Annals of Translational Medicine·Daniel A Zuppo, Michael Tsang
Jan 21, 2019·Neuropeptides·Scott P LevickJoseph S Janicki

❮ Previous
Next ❯

Methods Mentioned

BETA
Confocal microscopy
PCR
ELISA
reverse-transcription PCR

Software Mentioned

GraphPad Prism
GraphPad
ZEN
ZEISS
ImageJ

Related Concepts

Related Feeds

Adult Stem Cells

Adult stem cells reside in unique niches that provide vital cues for their survival, self-renewal, and differentiation. They hold great promise for use in tissue repair and regeneration as a novel therapeutic strategies. Here is the latest research.

Cell Migration

Cell migration is involved in a variety of physiological and pathological processes such as embryonic development, cancer metastasis, blood vessel formation and remoulding, tissue regeneration, immune surveillance and inflammation. Here is the latest research.

Cardiac Regeneration

Cardiac regeneration enables the repair of irreversibly damaged heart tissue using cutting-edge science, including stem cell and cell-free therapy. Discover the latest research on cardiac regeneration here.