Substance P-induced inward current in identified auditory efferent neurons in rat brain stem slices

Journal of Neurophysiology
X Wang, D Robertson

Abstract

The effects of substance P (SP) on whole cell currents were studied in neurons of the medial olivocochlear efferent system (MOCS) in the ventral nucleus of the trapezoid body (VNTB) of brain stem slices from neonatal rats. Each neuron was identified by retrograde labeling with Fast Blue injected into the cochlea. Bath application of SP (0.1-10 microM) reversibly induced an apparent inward current in 49 of 63 labeled neurons when voltage clamped at near resting voltages. This apparent inward current was consistent with the SP-induced membrane depolarization observed in current-clamp mode. The SP-induced change in current was dose dependent with a half-maximal response dose of 200 nM. It was mimicked by [Cys3,6, Tyr8, Pro9]-SP, a neurokinin (NK1) receptor selective agonist, whereas [Succinyl-Asp6, MePhe8]-SP 6-11 (Senktide), a NK3 receptor agonist, had no detectable effect. The SP effect was not blocked by 10(-6) M tetrodotoxin (TTX) and persisted when the perfusate contained 30 mM tetraethylammonium (TEA) or 100 microM Cd2+ or was in a 0-Ca solution. In a TTX-containing solution, SP caused a voltage-dependent decrease of membrane conductance, and the SP-evoked current reversed at a potential at around -105 mV. The predicted K+ e...Continue Reading

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Citations

Mar 11, 2000·The European Journal of Neuroscience·M Kouznetsova, A Nistri
Nov 9, 2000·Microscopy Research and Technique·A M Thompson, B R Schofield
Jun 15, 2006·The Journal of Membrane Biology·D DulonR L Davis
Jul 21, 2009·Clinical and Experimental Pharmacology & Physiology·Donald Robertson
Sep 30, 2000·Brain Research. Brain Research Reviews·H EndepolsH Luksch

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