Substance P potentiates 5-HT3 receptor-mediated current in rat trigeminal ganglion neurons

Neuroscience Letters
Wang-ping HuZhi-wang Li

Abstract

The present study aimed to investigate the interaction between the coexistent SP receptor and 5-HT3 receptor in trigeminal ganglion (TG) neurons using whole-cell patch clamp technique. The majority of the neurons examined responded to 5-HT with an inward current (I5-HT) (78.2%, 79/101) that could be blocked by 5-HT3 receptor antagonist, ICS-205,930. The I5-HT was potentiated by preapplication of SP (10(-10) to 10(-8) M) in most 5-HT-sensitive cells(78.5%, 62/79). Coapplication of SP and GR-82334, antagonist of NK1 receptor, had no enhancing effect on I5-HT. The concentration-response curves for 5-HT with and without SP preapplication show that: (1) the threshold 5-HT concentrations with and without SP preapplication are basically the same, while SP preapplication increased the maximal value of I5-HT by 38.0% of its control; (2) the EC50 values of the curves with and without SP pretreatment are very close, i.e. 1.89 x 10(-5) M and 2.08 x 10(-5) M (P > 0.1; n = 9), respectively. Intracellular dialysis of GDP-beta-S, a non-hydrolyzable GDP analog, and GF-109203X, a selective protein kinase C inhibitor, removed the SP potentiation of I5-HT. These results may offer a clue to understanding the mechanism underlying the generation and/...Continue Reading

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Citations

Mar 23, 2005·Acta Pharmacologica Sinica·Wang-ping HuZhi-wang Li
Aug 21, 2010·The Journal of Pharmacology and Experimental Therapeutics·Camilo RojasBarbara S Slusher
Nov 5, 2013·European Journal of Pharmacology·Camilo RojasBarbara S Slusher
Jun 17, 2010·Supportive Care in Cancer : Official Journal of the Multinational Association of Supportive Care in Cancer·Flavia LongoMarisa Di Seri
Jun 28, 2014·Experimental Brain Research·Ajit G ThomasBarbara S Slusher
Oct 13, 2009·European Journal of Pharmacology·Katharina HeimesEugen J Verspohl
Jul 25, 2007·International Endodontic Journal·J E WellsE K Proctor
Feb 13, 2018·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Huali WuJing Shang
Aug 1, 2014·The Journal of Pharmacology and Experimental Therapeutics·Beverley Greenwood-Van MeerveldAnthony Dickenson
Mar 19, 2013·Expert Opinion on Pharmacotherapy·Michelle C JanelsinsGary R Morrow
Aug 30, 2013·Expert Review of Anticancer Therapy·Sherry Mori-Vogt, Marlo Blazer
Sep 29, 2020·Frontiers in Pharmacology·Eslam El NebrisiMurat Oz
Jan 31, 2021·Supportive Care in Cancer : Official Journal of the Multinational Association of Supportive Care in Cancer·Sakiko KimuraHiroyuki Irie

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