Substance P potentiates ATP-activated currents in rat primary sensory neurons

Brain Research
H Z Hu, Z W Li

Abstract

The aim of this study was to explore whether substance P could modulate the response mediated by ATP receptor. Experiments were carried out on rat dorsal root ganglion (DRG) neurons isolated acutely with enzymatic and mechanical treatment. The ATP-activated inward currents were recorded using the whole-cell patch-clamp technique. The majority of the neurons examined (82/85, 96.5%) were sensitive to ATP (1-1000 microM). Application of substance P (0.01-100 microM) also caused an inward current. Several differences between these two kinds of currents were observed. 0.01, 0.1 and 1 microM substance P increased the ATP (10 microM)-activated current to 113.7 +/- 3.1%, (n = 8); 127.2 +/- 6.7%, (n = 12) and 154.7 +/- 14.4% (n = 6) (means +/- S.E.M.), respectively. This potentiating effect can be blocked by spantide, an NK1 receptor antagonist, and intracellular application of H7 (which is a potent inhibitor of PKC) could also block this kind of potentiation of SP on ATP-activated current. Since the substance P receptor and ATP receptor can coexist in rat DRG neurons and activation of substance P receptor can modulate the response mediated by ATP receptor, it suggests that they may cooperate with each other in activating peripheral noc...Continue Reading

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Citations

Apr 12, 2008·Purinergic Signalling·Kazuhide Inoue
Apr 25, 2018·Frontiers in Pharmacology·Xi ZhouZhonghua Liu
Mar 27, 2001·The Journal of Biological Chemistry·M PaukertS Gründer
May 5, 2005·Canadian Journal of Physiology and Pharmacology·Guo-Hua LiZhi-Wang Li
May 13, 1999·Journal of Neurophysiology·K XiongC Li
Jul 24, 2010·Journal of Dental Research·C K ParkS B Oh
Feb 13, 1999·Progress in Neurobiology·M J Millan

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