Substance P regulates the expression of matrix metalloproteinases and tissue inhibitors of metalloproteinase in cultured human gingival fibroblasts

Journal of Periodontal Research
Patricia R CuryN S de Araújo

Abstract

Substance P may play a role in the pathogenesis of periodontal disease; however, its mechanisms of modulation are not clear. This study evaluated the effect of two concentrations of Substance P on the expression of matrix metalloproteinases (MMPs) and tissue inhibitors of metalloproteinases (TIMPs) in cultured human gingival fibroblasts. Fibroblasts were stimulated for 48 h with 10(-4) or 10(-9) m Substance P; untreated fibroblasts served as controls. The expression of MMP-1, -2, -3, -7 and -11 and of TIMP-1 and -2 was evaluated using real-time polymerase chain reaction and western blotting. There was a significant, concentration-dependent stimulatory effect of Substance P on MMP-1, -2, -3 and -7 and TIMP-2 gene expression (p < 0.05), and a probable effect on MMP-11 (p = 0.06). At the higher concentration (10(-4) m Substance P), MMP-1, -2, -3, -7 and -11 and TIMP-2 showed the greatest up-regulation; at the lower concentration (10(-9) m Substance P), MMP-1, -3 and -7 and TIMP-2 exhibited diminished up-regulation, with MMP-2 and -11 showing down-regulation (p < 0.05). Expression of TIMP-1 was not affected by Substance P (p > 0.05). Western blotting confirmed that Substance P up-regulated MMP-1, -2, -3 and -11 and TIMP-2. MMP-1, -...Continue Reading

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Citations

Aug 19, 2008·Journal of Gastrointestinal Surgery : Official Journal of the Society for Surgery of the Alimentary Tract·Rizal LimJames M Becker
Sep 13, 2011·Cardiovascular Research·Giselle C MeléndezScott P Levick
Aug 9, 2011·Sports Medicine and Arthroscopy Review·Raffaele GarofaloAlessandro Castagna
Jan 17, 2012·Journal of Shoulder and Elbow Surgery·Angelo Del BuonoNicola Maffulli
Dec 17, 2008·Annals of the New York Academy of Sciences·Karen L ReedJames M Becker
Dec 21, 2012·Journal of Investigative and Clinical Dentistry·Shabnam Soltani BozchaloeiMahvash Oskoui
Aug 14, 2012·Journal of Oral Rehabilitation·A AkcaliN Buduneli
Nov 30, 2013·International Journal of Cardiology·Heather M Dehlin, Scott P Levick
Mar 31, 2016·Wound Repair and Regeneration : Official Publication of the Wound Healing Society [and] the European Tissue Repair Society·Ryan S ChiangAlan D Widgerow
Jun 18, 2016·Cellular and Molecular Life Sciences : CMLS·Alireza MashaghiReza Dana
Jun 10, 2017·International Journal of Molecular Sciences·Soo-Hong HanSoonchul Lee
Feb 6, 2018·Dermatologic Surgery : Official Publication for American Society for Dermatologic Surgery [et Al.]·Evan AustinJared Jagdeo
Jun 2, 2010·The Journal of Bone and Joint Surgery. British Volume·M J KhattakM Ahmed

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