Substance P stimulates release of RANKL via COX-2 expression in human dental pulp cells

Inflammation Research : Official Journal of the European Histamine Research Society ... [et Al.]
T KojimaK Kasai

Abstract

Our previous study found that substance P (SP), a sensory neuropeptide, was expressed in the dental pulp of rats during experimental tooth movement. We examined the effects of SP on the production of prostaglandin (PG) E2 and the receptor activator of nuclear factor- B ligand (RANKL) by human dental pulp fi broblast-like (HDPF) cells. SP was added to cultured HDPF cells at concentrations ranging from of 10(-4) to 10(-12) mol/L. PGE2 and soluble RANKL (sRANKL) levels were determined using enzyme-linked immunosorbent assay kits. Gene expression was confi rmed by RT-PCR analysis. Pit formation assays using dentin slices were carried out to examine the effect of SP on osteoclastogenesis. The levels of PGE2 and sRANKL increased in the presence of SP, though the increases were greater in the experimental groups in both a time- and concentration-dependent manner, and the increase of RANKL was partially mediated by PGE2. The gene expression of cyclooxygenase (COX)-2 and RANKL was up-regulated, and conditioned medium samples obtained from HDPF cells treated with SP induced bone resorption. SP stimulated the production of PGE2 and RANKL, and promoted bone resorption. Therefore, SP may be involved in pulpal inflammation and root resorptio...Continue Reading

Citations

Apr 5, 2012·Mediators of Inflammation·Paola Sacerdote, Luca Levrini
Nov 21, 2012·Journal of Dental Research·B GaoY P Li
Nov 7, 2013·Cell and Tissue Research·Piero RömerPeter Proff
Feb 24, 2016·Phytomedicine : International Journal of Phytotherapy and Phytopharmacology·Yumei LinKevin W Gellenbeck
Jul 28, 2007·Neuropeptides·Da LiuLi-Yang Dai
Jan 22, 2009·British Journal of Pharmacology·M GallicchioR Fantozzi
Jun 12, 2010·Journal of Orthopaedic Research : Official Publication of the Orthopaedic Research Society·Jian LiAndris Kreicbergs
Aug 21, 2012·International Endodontic Journal·G N BelibasakisM Zehnder
Feb 28, 2014·Journal of Investigative and Clinical Dentistry·Erica Dorigatti de AvilaCinara Maria Camparis
Aug 4, 2015·American Journal of Orthodontics and Dentofacial Orthopedics : Official Publication of the American Association of Orthodontists, Its Constituent Societies, and the American Board of Orthodontics·Yoko NakanoKazutaka Kasai
Jul 27, 2014·Matrix Biology : Journal of the International Society for Matrix Biology·Tanja NiedermairSusanne Grässel
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Apr 24, 2007·Journal of Biomedical Materials Research. Part a·Yebin QianYao Jiang
Aug 15, 2019·American Journal of Physiology. Cell Physiology·Hollie Allison, Laoise M McNamara

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