Substantia Gelatinosa neurons in defined-medium organotypic slice culture are similar to those in acute slices from young adult rats

Pain
Van B LuPeter A Smith

Abstract

Peripheral nerve injury promotes an enduring increase in the excitability of the spinal dorsal horn. This change, that likely underlies the development of chronic pain, may be a consequence of prolonged exposure of dorsal horn neurons to mediators such as neurotrophins, cytokines, and neurotransmitters. The long-term effects of such mediators can be analyzed by applying them to spinal neurons in organotypic slice culture. To assess the validity of this approach, we established serum-free, defined-medium organotypic cultures (DMOTC) from E13-14 prenatal rats. Whole-cell recordings were made from neurons maintained in DMOTC for up to 42 days. These were compared with recordings from neurons of similar age in acute spinal cord slices from 15- to 45-day-old rats. Five cell types were defined in acute slices as 'Tonic', 'Irregular', 'Delay', 'Transient' or 'Phasic' according to their discharge patterns in response to depolarizing current. Although fewer 'Phasic' cells were found in cultures, the proportions of 'Tonic', 'Irregular', 'Delay', and 'Transient' were similar to those found in acute slices. GABAergic, glycinergic, and 'mixed' inhibition were observed in neurons in acute slices and DMOTC. Pure glycinergic inhibition was abs...Continue Reading

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Citations

Apr 18, 2012·Canadian Journal of Physiology and Pharmacology·Van B LuSaifee Rashiq
Apr 19, 2015·Molecular Pain·Melissa A TadrosRobert J Callister
Aug 11, 2007·The Journal of Physiology·B A GrahamR J Callister
Jun 3, 2008·The European Journal of Neuroscience·Brenda Bonnici, Josef P Kapfhammer
Oct 11, 2016·Scientific Reports·Maria Amalia Di CastroCristina Limatola
Apr 28, 2016·The Neuroscientist : a Review Journal Bringing Neurobiology, Neurology and Psychiatry·Sascha R A Alles, Peter A Smith
Feb 22, 2008·Journal of Neurophysiology·B A GrahamR J Callister
Mar 4, 2018·Pharmacological Reviews·Sascha R A Alles, Peter A Smith
Sep 4, 2015·Journal of Neurophysiology·Melissa Anne TadrosRobert J Callister
Jan 28, 2021·Scientific Reports·Sascha R A AllesPeter A Smith
Jun 29, 2021·Frontiers in Neuroscience·Paula P PerissinottiErika S Piedras-Rentería

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