Substantial improvement of toyocamycin production in Streptomyces diastatochromogenes by cumulative drug-resistance mutations

PloS One
Xuping ShentuXiaoping Yu

Abstract

Toyocamycin is a member of the nucleoside antibiotic family and has been recognized as a promising fungicide for the control of plant diseases. However, low productivity of toyocamycin remains an important bottleneck in its industrial production. Therefore, dramatic improvements of strains for overproduction of toyocamycin are of great interest in applied microbiology research. In this study, we sequentially selected for mutations for multiple drug resistance to promote the overproduction of toyocamycin by Streptomyces diastatochromogenes 1628. The triple mutant strain, SD3145 (str str par), was obtained through sequential screenings. This strain showed an enhanced capacity to produce toyocamycin (1500 mg/L), 24-fold higher than the wild type in GYM liquid medium. This dramatic overproduction was attributed at least partially to the acquisition of an rsmG mutation and increased gene expression of toyA, which encodes a LuxR-family transcriptional regulator for toyocamycin biosynthesis. The expression of toyF and toyG, probably directly involved in toyocamycin biosynthesis, was also enhanced, contributing to toyocamycin overproduction. By addition of a small amount of scandium (ScCl3·6H2O), the mutant strain, SD3145, produced mor...Continue Reading

References

Oct 6, 1999·Current Opinion in Microbiology·D I Andersson, B R Levin
Nov 10, 2001·Archives of Microbiology·M G WatveB D Bhole
Nov 30, 2004·Advances in Applied Microbiology·Kozo OchiKazuhiko Kurosawa
Jun 26, 2007·Bioscience, Biotechnology, and Biochemistry·Kozo Ochi
Jul 25, 2007·FEMS Microbiology Letters·Keiichi KawaiKozo Ochi
Sep 12, 2007·Veterinary Pathology·R D JollyS U Walkley
Jun 13, 2008·Nature Protocols·Thomas D Schmittgen, Kenneth J Livak

❮ Previous
Next ❯

Methods Mentioned

BETA
amino acid exchange
PCR

Related Concepts

Related Feeds

Bacterial Protein Structures

Bacterial protein structures can expedite the development of novel antibiotics. Here is the latest research on bacterial proteins and the resolution of their structures.

Antimicrobial Resistance (ASM)

Antimicrobial resistance poses a significant threat to the continued successful use of antimicrobial agents for the treatment of bacterial infections.

Antimicrobial Resistance

Antimicrobial resistance poses a significant threat to the continued successful use of antimicrobial agents for the treatment of bacterial infections.