Substituted beta-cyclodextrins interact with PAMAM dendrimer-DNA complexes and modify transfection efficiency

Biochemical and Biophysical Research Communications
B J RoesslerJ R Baker

Abstract

The efficiency of PAMAM dendrimer-mediated DNA transfer can be improved by the addition of substituted beta-cyclodextrins (beta-CDs) as formulation excipients. In vitro CAT expression increased approximately 200-fold when dendrimer/DNA/beta-CD formulations were applied on the surface of collagen membranes. The inclusion of beta-CD into the formulations resulted in particles that were smaller and more evenly distributed on the surface of the solid support. The average size of the complex formed at 50 microg/ml and at charge ratio of 1 decreased from 156 nm to 5.8 nm and 21.2 nm in 0.025-0.1% w/vol beta-CDs. Sulfonated beta-CDs bind to dendrimer and in the increased concentration may displace DNA in the dendrimer/DNA complex. High concentrations of amphoteric beta-CD do not dissociate dendrimer/DNA complexes; however, they may decrease their ability to transfect cells. At the optimized formulations the surface-modified beta-CDs may enhance solid support-based transfection in vitro, through modification of dendrimer/DNA complex composition and improved surface distribution.

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Citations

May 16, 2014·Nanomedicine·Ariane TschicheRainer Haag
Apr 23, 2013·Advanced Drug Delivery Reviews·Hidetoshi ArimaTaishi Higashi
Dec 10, 2002·Current Opinion in Chemical Biology·Mary J Cloninger
Jul 3, 2013·European Journal of Pharmaceutics and Biopharmaceutics : Official Journal of Arbeitsgemeinschaft Für Pharmazeutische Verfahrenstechnik E.V·Cristina ArandaConchita Tros de Ilarduya
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Feb 7, 2012·International Journal of Pharmaceutics·Takayuki AnnoHidetoshi Arima
Nov 30, 2011·Nanomedicine·Alejandro Méndez-ArdoyConchita Tros de Ilarduya
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Nov 3, 2010·Chemical Society Reviews·Carmen Ortiz MelletJuan M Benito

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