PMID: 6540625Sep 1, 1984Paper

Substituting an alpha-helix switches the sequence-specific DNA interactions of a repressor

Cell
R P WhartonM Ptashne

Abstract

It has been suggested that many DNA-binding proteins use an alpha-helix for specific sequence recognition. We have used amino acid sequence homologies to identify the presumptive DNA-recognition helices in two related proteins whose structures are unknown--the repressor and cro protein of bacteriophage 434. The 434 repressor and cro protein each bind to three similar sites in the rightward phage 434 operator, OR, and they make different contacts in each binding site, as revealed by the chemical probe dimethyl sulfate. We substituted the putative recognition alpha-helix of 434 repressor with the putative recognition alpha-helix of 434 cro protein to create a hybrid protein named repressor*. The specific DNA contacts made by repressor* are like those of 434 cro protein.

References

Oct 1, 1979·Proceedings of the National Academy of Sciences of the United States of America·A D JohnsonM Ptashne
Feb 1, 1977·Proceedings of the National Academy of Sciences of the United States of America·A M Maxam, W Gilbert
Apr 1, 1978·Proceedings of the National Academy of Sciences of the United States of America·A JohnsonM Ptashne
Mar 1, 1979·Nucleic Acids Research·R Grosschedl, E Schwarz
May 1, 1982·Proceedings of the National Academy of Sciences of the United States of America·T A SteitzB W Matthews
May 1, 1983·Proceedings of the National Academy of Sciences of the United States of America·M H HechtR T Sauer
Oct 25, 1980·Journal of Molecular Biology·F SangerB A Roe
Jan 22, 1982·Nucleic Acids Research·A Kolb, H Buc
Jul 1, 1982·Proceedings of the National Academy of Sciences of the United States of America·D DiMaioT Maniatis
Mar 1, 1984·Proceedings of the National Academy of Sciences of the United States of America·J AndersonS C Harrison
Jul 1, 1984·Proceedings of the National Academy of Sciences of the United States of America·I T Weber, T A Steitz
Mar 1, 1982·Proceedings of the National Academy of Sciences of the United States of America·B W MatthewsY Takeda
Jul 1, 1981·Proceedings of the National Academy of Sciences of the United States of America·R Brent, M Ptashne

❮ Previous
Next ❯

Citations

May 20, 1991·Journal of Molecular Biology·A Mondragón, S C Harrison
Sep 1, 1989·Neurobiology of Aging·C B Caputo, A I Salama
Feb 1, 1988·Biophysical Chemistry·S C HarrisonM Ptashne
Jan 1, 1985·Bio Systems·W F Anderson, M Cygler
Jul 28, 1989·Biochimica Et Biophysica Acta·M P ScottG W Hartzell
Dec 10, 2003·Current Opinion in Biotechnology·Cynthia H CollinsFrances H Arnold
Oct 27, 1988·Nature·C WolbergerS C Harrison
Sep 5, 2001·Molecular Microbiology·T Shi, D I Friedman
Jun 1, 1985·Proceedings of the National Academy of Sciences of the United States of America·F Barany
Apr 1, 1985·Proceedings of the National Academy of Sciences of the United States of America·J L EliasonM Ptashne
Dec 1, 1986·Proceedings of the National Academy of Sciences of the United States of America·F D Bushman, M Ptashne
Aug 1, 1987·Proceedings of the National Academy of Sciences of the United States of America·J A WellsD A Estell
Aug 1, 1988·Proceedings of the National Academy of Sciences of the United States of America·M HollisM Ptashne
Aug 1, 1986·Journal of Biomolecular Structure & Dynamics·O B Ptitsyn, M V Volkenstein
Jun 1, 1986·Journal of Biomolecular Structure & Dynamics·M Cygler, W F Anderson
May 27, 1986·Nucleic Acids Research·A WissmannW Hillen
Feb 28, 1998·Nucleic Acids Research·G B Koudelka
Jul 6, 2006·Nucleic Acids Research·Marc van DijkAlexandre M J J Bonvin
Mar 7, 2009·Nucleic Acids Research·Tasha A DesaiChristopher V Rao
May 15, 2010·Nucleic Acids Research·Marc van Dijk, Alexandre M J J Bonvin
Apr 12, 2012·Nucleic Acids Research·Massimiliano Zampini, Finbarr Hayes
Feb 1, 1992·Molecular Microbiology·C WebsterW Brammar
Feb 20, 2007·Journal of Bacteriology·Paul Shkilnyj, Gerald B Koudelka
May 16, 2000·Journal of Bacteriology·J Xu, G B Koudelka
Dec 18, 2003·Journal of Bacteriology·David R Pawlowski, Gerald B Koudelka
Jun 8, 2001·Annual Review of Biochemistry·C O PaboR A Grant
Apr 18, 2012·PloS One·Tammy J BullwinkleGerald B Koudelka
Apr 1, 1988·Biological Chemistry Hoppe-Seyler·M SignoriniC M Bergamini
Jan 13, 2012·Current Opinion in Biotechnology·Christopher V Rao
Feb 20, 1991·Journal of Molecular Biology·R L SomervilleJ H Zeilstra-Ryalls

❮ Previous
Next ❯

Related Concepts

Related Feeds

Cell eTOC

Cell is a scientific journal publishing research across a broad range of disciplines within the life sciences field. Discover the latest research from Cell here.