PMID: 7535929Mar 28, 1995Paper

Substitution of a highly basic helix/loop sequence into the RNase H domain of human immunodeficiency virus reverse transcriptase restores its Mn(2+)-dependent RNase H activity

Proceedings of the National Academy of Sciences of the United States of America
J L Keck, S Marqusee

Abstract

Human immunodeficiency virus (HIV) reverse transcriptase (RT) is a multifunctional protein, containing both DNA polymerase and RNase H activity. The RNase H activity of HIV RT catalyzes the hydrolysis of the RNA strand of RNA.DNA hybrids. While the domain that carries out the RNase H activity in HIV RT can be expressed as an independent, folded polypeptide, it is inactive as an RNase H. Here, we report the overexpression and purification of an active, recombinant HIV RNase H domain in which the sequence corresponding to the Escherichia coli RNase H1 basic helix/loop has been substituted for the corresponding sequence of HIV RNase H. The resulting polypeptide (RNH102) has Mn(2+)-dependent RNase H activity and is more stable than the independently expressed wild-type HIV RNase H domain.

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Citations

Oct 29, 1998·Protein Science : a Publication of the Protein Society·G KernS Marqusee
Feb 1, 1995·Current Opinion in Structural Biology·E ArnoldZ Hostomsky
Apr 6, 2004·Biochemical and Biophysical Research Communications·Julie Qi HangKlaus Klumpp
May 30, 2002·Biophysical Chemistry·Gunther KernDorothee Kern
Jul 20, 2011·Antimicrobial Agents and Chemotherapy·Richard V FariasOscar Leon
Aug 17, 2006·Journal of Virology·David LimStephen P Goff
Oct 8, 2013·PLoS Computational Biology·Kate A StaffordArthur G Palmer
Apr 6, 2011·Antimicrobial Agents and Chemotherapy·Eric B LansdonThorsten A Kirschberg
Apr 2, 2010·Cellular and Molecular Life Sciences : CMLS·Alon Herschhorn, Amnon Hizi
Jan 3, 2016·Journal of Molecular Biology·Shilpa Yadahalli, Shachi Gosavi
Feb 12, 2008·Virus Research·Sharon J Schultz, James J Champoux
Aug 1, 2012·Chemical Biology & Drug Design·Martin T ChristenRieko Ishima
Dec 1, 2010·Chemical Biology & Drug Design·Qingguo GongRieko Ishima
Apr 1, 2010·Viruses·Greg L Beilhartz, Matthias Götte
Nov 18, 2000·The Journal of Biological Chemistry·E R Goedken, S Marqusee
Nov 8, 2006·Protein Science : a Publication of the Protein Society·Joel A Butterwick, Arthur G Palmer
May 3, 2012·Protein Science : a Publication of the Protein Society·Ashok SekharSilvia Cavagnero
Dec 16, 1998·The Journal of Biological Chemistry·J L KeckS Marqusee
Apr 10, 1999·The Journal of Biological Chemistry·S DruillennecB P Roques
Aug 16, 1996·The Journal of Biological Chemistry·J L Keck, S Marqusee
Dec 14, 2002·The Journal of Biological Chemistry·Cathryn A Shaw-ReidDaria J Hazuda
Oct 3, 2019·ACS Infectious Diseases·Zhaoyong XiRieko Ishima

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