PMID: 9422601Jan 9, 1998Paper

Substrate ambiguity of 3-deoxy-D-manno-octulosonate 8-phosphate synthase from Neisseria gonorrhoeae in the context of its membership in a protein family containing a subset of 3-deoxy-D-arabino-heptulosonate 7-phosphate synthases

Journal of Bacteriology
P S SubramaniamR A Jensen

Abstract

3-Deoxy-D-manno-octulosonate 8-phosphate (KDOP) synthase and 3-deoxy-D-arabino-heptulosonate 7-phosphate (DAHP) synthase catalyze similar phosphoenolpyruvate-utilizing reactions. The genome of Neisseria gonorrhoeae contains one gene encoding KDOP synthase and one gene encoding DAHP synthase. Of the two nonhomologous DAHP synthase families known, the N. gonorrhoeae protein belongs to the family I assemblage. KDOP synthase exhibited an ability to replace arabinose-5-P with either erythrose-4-P or ribose-5-P as alternative substrates. The results of periodate oxidation studies suggested that the product formed by KDOP synthase with erythrose-4-P as the substrate was 3-deoxy-D-ribo-heptulosonate 7-P, an isomer of DAHP. As expected, this product was not utilized as a substrate by dehydroquinate synthase. The significance of the ability of KDOP synthase to substitute erythrose-4-P for arabinose-5-P is (i) recognition of the possibility that the KDOP synthase might otherwise be mistaken for a species of DAHP synthase and (ii) the possibility that the broad-specificity type of KDOP synthase might be a relatively vulnerable target for antimicrobial agents which mimic the normal substrates. An analysis of sequences in the database indica...Continue Reading

References

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Citations

Nov 4, 2005·Organic & Biomolecular Chemistry·Meekyung AhnEmily J Parker
Sep 11, 2007·The Journal of Antibiotics·Neil P J Price, Billyana Tsvetanova
Jan 15, 1999·Critical Reviews in Microbiology·C M Kahler, D S Stephens
Mar 12, 2004·Annual Review of Plant Physiology and Plant Molecular Biology·Klaus M. Herrmann, Lisa M. Weaver
Jun 12, 2013·Protein Science : a Publication of the Protein Society·Penelope J CrossEmily J Parker
Feb 13, 2013·Protein Science : a Publication of the Protein Society·Samuel H Light, Wayne F Anderson
Jul 3, 2002·The Journal of Biological Chemistry·Billyana C TsvetanovaNeil P J Price
Sep 11, 2003·Microbiology and Molecular Biology Reviews : MMBR·Gary XieRoy A Jensen
Aug 3, 2020·Current Opinion in Structural Biology·Wanting JiaoEmily J Parker
Feb 6, 2021·Biochemical Society Transactions·Wanting Jiao
Jan 16, 2002·Comparative Biochemistry and Physiology. Toxicology & Pharmacology : CBP·G XieR A Jensen
Sep 1, 2008·EcoSal Plus·James Pittard, Ji Yang
May 19, 2009·Journal of Molecular Biology·Fiona C CochraneEmily J Parker

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