PMID: 6952227Mar 1, 1982

Substrate binding affinity changes in mitochondrial energy-linked reactions

Proceedings of the National Academy of Sciences of the United States of America
Y HatefiY M Galante

Abstract

The effects of uncouplers and valinomycin plus nigericin (in the presence of K+) were studied on the apparent Km for substrates and apparent Vmax of the following energy-linked reactions catalyzed by submitochondrial particles: oxidative phosphorylation, NTP-33Pi exchange, ATP-driven electron transfer from succinate to NAD, and respiration-driven transhydrogenation from NADH to 3-acetylpyridine adenine dinucleotide phosphate. In all cases, partially uncoupling (up to 90%) concentrations of uncouplers of valinomycin plus nigericin were found to decrease apparent Vmax and to increase apparent Km. Results plotted as ln (Vmax/Km) versus the concentration of uncouplers or ionophores showed a linear decrease of the former as a function of increasing perturbant concentration (i.e., decreasing free energy). Because Vmax/Km may be considered as a measure of the apparent first-order rate constant for enzyme-substrate interaction and reflects the affinity between enzyme and substrate to form a complex, the results are consistent with the interpretation that membrane energization leads to a change in enzyme conformation with the resultant increase in enzyme-substrate affinity and facilitation of the reaction rate under consideration. The s...Continue Reading

References

Mar 1, 1977·Proceedings of the National Academy of Sciences of the United States of America·Y Hatefi, Y M Galante
Jul 3, 1979·Biochimica Et Biophysica Acta·B A Baird, G G Hammes
Jul 9, 1976·Biochimica Et Biophysica Acta·G Dooijewaard, E C Slater
Jul 9, 1976·Biochimica Et Biophysica Acta·G Dooijewaard, E C Slater
Oct 4, 1976·Biochemical and Biophysical Research Communications·C KayalarP D Boyer
Jun 28, 1973·Biochimica Et Biophysica Acta·R M BertinaE C Slater
Aug 1, 1973·Archives of Biochemistry and Biophysics·M ErecińskaB Chance
Apr 8, 1970·Biochemical and Biophysical Research Communications·D F Wilson, P L Dutton
Feb 1, 1970·Archives of Biochemistry and Biophysics·D F Wilson, P L Dutton
Apr 30, 1981·Biochemical and Biophysical Research Communications·C Vinkler

Citations

Aug 1, 1988·Journal of Bioenergetics and Biomembranes·A Matsuno-Yagi, Y Hatefi
Feb 1, 1992·Journal of Bioenergetics and Biomembranes·R AlvaS Estrada
Nov 1, 1985·Proceedings of the National Academy of Sciences of the United States of America·A Matsuno-YagiY Hatefi
Feb 1, 1985·European Journal of Biochemistry·A L MargolinV A Kabanov
Sep 1, 1986·European Journal of Biochemistry·M Rögner, Peter Gräber
Dec 21, 2010·Biochemistry. Biokhimii︠a︡·K A KegyarikovaA D Vinogradov
Jun 15, 2007·Biophysical Chemistry·Bernard Korzeniewski
Aug 10, 2010·Biochimica Et Biophysica Acta·Kathrin FörsterJan Petersen
Jun 28, 2002·The Journal of Biological Chemistry·Mutsuo YamaguchiY Hatefi
Dec 15, 1993·European Journal of Biochemistry·Y Hatefi
Feb 25, 2006·Human Molecular Genetics·Ting-Ting HuangCharles J Epstein

Related Concepts

Adenosine Triphosphate, Chromium Ammonium Salt
Bos indicus
Cell-Free System
Energy Metabolism
Mitochondria, Heart
NADH
Pandavir
Nucleotides
Oxidative Phosphorylation
Substrate Specificity

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Alzheimer's Disease: MS4A

Variants within the membrane-spanning 4-domains subfamily A (MS4A) gene cluster have recently been implicated in Alzheimer's disease in genome-wide association studies. Here is the latest research on Alzheimer's disease and MS4A.

Pediculosis pubis

Pediculosis pubis is a disease caused by a parasitic insect known as Pthirus pubis, which infests human pubic hair, as well as other areas with hair including eye lashes. Here is the latest research.

Rh Isoimmunization

Rh isoimmunization is a potentially preventable condition that occasionally is associated with significant perinatal morbidity or mortality. Discover the latest research on Rh Isoimmunization here.

Genetic Screens in iPSC-derived Brain Cells

Genetic screening is a critical tool that can be employed to define and understand gene function and interaction. This feed focuses on genetic screens conducted using induced pluripotent stem cell (iPSC)-derived brain cells. It also follows CRISPR-Cas9 approaches to generating genetic mutants as a means of understanding the effect of genetics on phenotype.

Enzyme Evolution

This feed focuses on molecular models of enzyme evolution and new approaches (such as adaptive laboratory evolution) to metabolic engineering of microorganisms. Here is the latest research.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Pharmacology of Proteinopathies

This feed focuses on the pharmacology of proteinopathies - diseases in which proteins abnormally aggregate (i.e. Alzheimer’s, Parkinson’s, etc.). Discover the latest research in this field with this feed.

Alignment-free Sequence Analysis Tools

Alignment-free sequence analyses have been applied to problems ranging from whole-genome phylogeny to the classification of protein families, identification of horizontally transferred genes, and detection of recombined sequences. Here is the latest research.