Substrate binding to BamD triggers a conformational change in BamA to control membrane insertion

Proceedings of the National Academy of Sciences of the United States of America
James LeeDaniel Kahne

Abstract

The β-barrel assembly machine (Bam) complex folds and inserts integral membrane proteins into the outer membrane of Gram-negative bacteria. The two essential components of the complex, BamA and BamD, both interact with substrates, but how the two coordinate with each other during assembly is not clear. To elucidate aspects of this process we slowed the assembly of an essential β-barrel substrate of the Bam complex, LptD, by changing a conserved residue near the C terminus. This defective substrate is recruited to the Bam complex via BamD but is unable to integrate into the membrane efficiently. Changes in the extracellular loops of BamA partially restore assembly kinetics, implying that BamA fails to engage this defective substrate. We conclude that substrate binding to BamD activates BamA by regulating extracellular loop interactions for folding and membrane integration.

References

Jul 5, 1976·Journal of Molecular Biology·M J Casadaban
Mar 1, 1996·Journal of Bacteriology·S W Lazar, R Kolter
Aug 3, 2002·Proceedings of the National Academy of Sciences of the United States of America·Jason W ChinPeter G Schultz
Sep 2, 2003·Current Opinion in Structural Biology·William C Wimley
Nov 24, 2005·Proceedings of the National Academy of Sciences of the United States of America·Daniel D IsaacThomas J Silhavy
Jul 11, 2006·Molecular Microbiology·Juliana C MalinverniThomas J Silhavy
Jul 25, 2006·Proceedings of the National Academy of Sciences of the United States of America·Tao WuDaniel Kahne
Apr 4, 2007·Proceedings of the National Academy of Sciences of the United States of America·Joseph G SklarThomas J Silhavy
Oct 3, 2007·Genes & Development·Joseph G SklarThomas J Silhavy
Mar 25, 2008·Cell·Stephan KutikNils Wiedemann
Mar 29, 2008·Methods in Molecular Biology·Mark P Molloy
Apr 1, 2008·Proceedings of the National Academy of Sciences of the United States of America·Natividad RuizThomas J Silhavy
Apr 23, 2009·Proceedings of the National Academy of Sciences of the United States of America·Juliana C Malinverni, Thomas J Silhavy
Mar 6, 2010·Proceedings of the National Academy of Sciences of the United States of America·Shu-Sin ChngDaniel Kahne
Apr 10, 2010·Science·Christine L HaganDaniel Kahne
Jun 23, 2010·Proceedings of the National Academy of Sciences of the United States of America·Natividad RuizThomas J Silhavy
Jan 25, 2011·Proceedings of the National Academy of Sciences of the United States of America·Elizaveta FreinkmanDaniel Kahne
Apr 6, 2011·Journal of Molecular Biology·Cristina M SandovalMarcelo C Sousa
May 19, 2011·The Journal of Biological Chemistry·Reinhard Albrecht, Kornelius Zeth
Jul 2, 2011·Current Opinion in Structural Biology·James W FairmanSusan K Buchanan
Dec 20, 2011·Journal of Bacteriology·Nathan W RigelThomas J Silhavy
Feb 15, 2012·Proceedings of the National Academy of Sciences of the United States of America·Dante P RicciThomas J Silhavy
Mar 13, 2013·Proceedings of the National Academy of Sciences of the United States of America·Nathan W RigelThomas J Silhavy
Aug 8, 2013·Biochemistry·Christine L HaganDaniel Kahne
Sep 3, 2013·Nature·Nicholas NoinajSusan K Buchanan
Sep 5, 2013·Proceedings of the National Academy of Sciences of the United States of America·Shin-ichiro NaritaYoshinori Akiyama
Jul 2, 2014·Structure·Nicholas NoinajSusan K Buchanan
Jan 9, 2016·Science·Jeremy BakelarNicholas Noinaj
Feb 24, 2016·Nature Structural & Molecular Biology·Long HanYihua Huang
Feb 24, 2016·Nature·Yinghong GuChangjiang Dong
May 11, 2016·Journal of Bacteriology·Tara F MahoneyThomas J Silhavy
Jul 22, 2016·Proceedings of the National Academy of Sciences of the United States of America·James LeeDaniel E Kahne
Aug 3, 2016·Proceedings of the National Academy of Sciences of the United States of America·Shawn M CostelloKaren G Fleming
Feb 23, 2017·Proceedings of the National Academy of Sciences of the United States of America·Joseph S WzorekDaniel E Kahne

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Citations

Aug 8, 2018·FEMS Microbiology Letters·Peter van UlsenJoen Luirink
Mar 13, 2019·Journal of Bacteriology·Elizabeth M HartThomas J Silhavy
Jun 20, 2019·Frontiers in Microbiology·Ina MeuskensDirk Linke
Oct 28, 2019·Nature·Anatol LutherDaniel Obrecht
Jun 6, 2020·The Journal of Biological Chemistry·Jim E HorneSheena E Radford
Jul 6, 2020·Nature Communications·Kathryn A DiederichsSusan K Buchanan
Jul 18, 2020·Proceedings of the National Academy of Sciences of the United States of America·Elizabeth M HartThomas J Silhavy
Oct 9, 2019·Proceedings of the National Academy of Sciences of the United States of America·Elizabeth M HartThomas J Silhavy
Aug 9, 2019·The Journal of Biological Chemistry·John J PsonisDavid G Thanassi
Dec 20, 2018·Frontiers in Immunology·Benjamin I BaardaAleksandra E Sikora
May 5, 2018·FEMS Microbiology Letters·David RanavaRaffaele Ieva
Aug 21, 2020·Journal of Bacteriology·Elizabeth M Hart, Thomas J Silhavy
Dec 15, 2020·Molecular Microbiology·Karl LundquistNicholas Noinaj
Dec 16, 2020·Communications Biology·Matthew G IadanzaNeil A Ranson
Feb 10, 2021·Current Opinion in Microbiology·David Tomasek, Daniel Kahne
Feb 28, 2021·Journal of Molecular Biology·Kathryn A DiederichsIstvan Botos
Sep 22, 2020·Chemical Reviews·Emily LundstedtNatividad Ruiz
Mar 15, 2019·EcoSal Plus·Dante P Ricci, Thomas J Silhavy
May 29, 2021·FEMS Microbiology Letters·Maurice SteenhuisJoen Luirink
Jul 9, 2021·Nature Communications·Paul WhiteSheena E Radford
Aug 6, 2021·Proceedings of the National Academy of Sciences of the United States of America·Muralidhar TataAnna Konovalova
Jan 1, 2022·Proceedings of the National Academy of Sciences of the United States of America·Ashton N Combs, Thomas J Silhavy

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