Substrate elasticity modulates TGF beta stimulated re-differentiation of expanded human articular chondrocytes

Drug Delivery and Translational Research
Daniel VonwilIvan Martin

Abstract

Substrate elasticity has emerged as important biomaterial design parameter. In particular, it has been reported that on soft substrates (~4 kPa) freshly isolated porcine chondrocytes better maintain their phenotype than on stiffer ones (>20 kPa). Thus, we investigated whether this also applies to re-differentiating, expanded/de-differentiated (EDD) human articular chondrocytes (HAC). EDD HAC were seeded onto Type I collagen functionalized poly acrylamide (PA) films with a Young's modulus of 0.26 ± 0.08 kPa (soft), 21.32 ± 0.79 kPa (intermediately stiff) and 74.88 ± 5.13 kPa (stiff), or type I collagen-coated plastic dishes (TCPS w/CI). Cells were cultured for 7 to 14 days in chondrogenic medium supplemented with transforming growth factor beta-1 (TGF-β1) and assessed for attachment, initial adhesion strength, proliferation, morphology as well as for expression of type I and II collagen at mRNA and type II collagen on protein level. Attachment and adhesion strength was similar on the different PA substrates and proliferation remained marginal (<1 doubling/week). On intermediately stiff to infinitely stiff substrates EDD HAC assumed a spindle shaped, fibroblastic morphology, whereas on the soft substrate they remained more spheri...Continue Reading

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Citations

May 20, 2020·Frontiers in Bioengineering and Biotechnology·Barbara BachmannPeter Ertl

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