Substrate elasticity modulates the responsiveness of mesenchymal stem cells to commitment cues

Integrative Biology : Quantitative Biosciences From Nano to Macro
S GobaaM P Lutolf

Abstract

Fate choices of stem cells are regulated in response to a complex array of biochemical and physical signals from their microenvironmental niche. Whereas the molecular composition and the role of mechanical niche cues have been extensively studied, relatively little is known about how both effectors act in concert to modulate stem cell fate. Here we utilized a recently developed artificial niche microarray platform to investigate whether the stiffness of a cell culture substrate influences how niche signaling factors exert their role on adipogenic differentiation of human mesenchymal stem cells (hMSC). We found that substrate stiffness imposes a strictly non-overlapping range of differentiation, highlighting the dominance of physical over the biochemical factors. At a given stiffness, a significant protein-dependent effect on adipogenic differentiation was observed. Furthermore, we show that synergistic interactions between proteins can also be driven by the substrate stiffness. Our results thus highlight the importance of considering the mechanical properties of a target tissue when investigating biochemical niche signals in vitro.

References

Aug 1, 1988·Proceedings of the National Academy of Sciences of the United States of America·F M WattC H O'Neill
Apr 10, 1999·Ultrasonic Imaging·T A KrouskopT Hall
Sep 1, 1999·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·F GoriB L Riggs
Sep 4, 2004·Stem Cells and Development·Adel Alhadlaq, Jeremy J Mao
Oct 16, 2004·Journal of Cellular Biochemistry·Genevieve M BolandRocky S Tuan
Aug 23, 2006·Cell·Adam J EnglerDennis E Discher
May 8, 2007·ACS Chemical Biology·Ratmir DerdaLaura L Kiessling
Feb 15, 2008·Stem Cells and Development·Christopher J FlaimSangeeta N Bhatia
Sep 2, 2008·Oncogene·B D'SouzaG Weinmaster
Jun 27, 2009·Science·Dennis E DischerPeter W Zandstra
Jul 3, 2009·Cell Stem Cell·Farshid GuilakChristopher S Chen
Jul 22, 2009·Proceedings of the National Academy of Sciences of the United States of America·Haiyan HuangQi-Qun Tang
Sep 22, 2009·European Cells & Materials·Nicholas D EvansMolly M Stevens
Dec 22, 2009·Integrative Biology : Quantitative Biosciences From Nano to Macro·Mark A LaBargeMina J Bissell
Sep 29, 2011·Proceedings of the National Academy of Sciences of the United States of America·Hemant V UnadkatJan de Boer
Oct 11, 2011·Nature Methods·Samy GobaaMatthias P Lutolf
Mar 13, 2012·Annual Review of Biophysics·Yubing SunJianping Fu
May 23, 2012·Current Opinion in Genetics & Development·Edward Giniger
May 29, 2012·Nature Materials·Britta TrappmannWilhelm T S Huck
May 3, 2013·Journal of the Royal Society, Interface·Luke MacQueenCraig A Simmons
Oct 10, 2013·American Journal of Physiology. Endocrinology and Metabolism·Nadia AlkhouliC Peter Winlove

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Citations

Aug 16, 2016·Current Opinion in Solid State & Materials Science·Gillie Agmon, Karen L Christman
Jun 22, 2016·Current Molecular Biology Reports·Gunes UzerJanet Rubin
Apr 7, 2016·ACS Applied Materials & Interfaces·Kelly M MabryKristi S Anseth
Apr 12, 2016·Journal of Tissue Engineering and Regenerative Medicine·Rossella CalabreseDavid L Kaplan
Jun 16, 2015·Integrative Biology : Quantitative Biosciences From Nano to Macro·Christopher Moraes
Jul 28, 2016·Cell Stem Cell·Laralynne PrzybylaValerie M Weaver
Mar 5, 2017·Journal of the American Chemical Society·Akiko NihoYuki Sudo
Apr 8, 2017·Current Opinion in Biotechnology·Tina TronserPavel A Levkin
Jan 13, 2018·Annual Review of Biomedical Engineering·Pamela K KreegerKristyn S Masters
Jan 7, 2016·Journal of Materials Chemistry. B, Materials for Biology and Medicine·Fengxuan HanBin Li
Jan 26, 2021·Frontiers in Bioengineering and Biotechnology·Fabrizio A PennacchioPaolo Maiuri
Feb 12, 2018·ACS Biomaterials Science & Engineering·Elizabeth A BrooksShelly R Peyton

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