Substrate elasticity regulates skeletal muscle stem cell self-renewal in culture.

Science
P M GilbertH M Blau

Abstract

Stem cells that naturally reside in adult tissues, such as muscle stem cells (MuSCs), exhibit robust regenerative capacity in vivo that is rapidly lost in culture. Using a bioengineered substrate to recapitulate key biophysical and biochemical niche features in conjunction with a highly automated single-cell tracking algorithm, we show that substrate elasticity is a potent regulator of MuSC fate in culture. Unlike MuSCs on rigid plastic dishes (approximately 10(6) kilopascals), MuSCs cultured on soft hydrogel substrates that mimic the elasticity of muscle (12 kilopascals) self-renew in vitro and contribute extensively to muscle regeneration when subsequently transplanted into mice and assayed histologically and quantitatively by noninvasive bioluminescence imaging. Our studies provide novel evidence that by recapitulating physiological tissue rigidity, propagation of adult muscle stem cells is possible, enabling future cell-based therapies for muscle-wasting diseases.

References

Jan 1, 1991·Muscle & Nerve·S WakefordT A Partridge
Jul 1, 1996·Developmental Dynamics : an Official Publication of the American Association of Anatomists·S TajbakhshM Buckingham
Feb 12, 1998·Proceedings of the National Academy of Sciences of the United States of America·R J Pelham, Y l Wang
May 22, 2002·The Journal of Cell Biology·Zhuqing Qu-PetersenJohnny Huard
Dec 23, 2003·Proceedings of the National Academy of Sciences of the United States of America·Yu-An CaoChristopher H Contag
Jul 28, 2004·The Journal of Cell Biology·Peter S ZammitJonathan R Beauchamp
Sep 6, 2005·Science·Didier MontarrasMargaret Buckingham
Jun 27, 2006·Nature Cell Biology·Vasily ShininShahragim Tajbakhsh
Aug 23, 2006·Cell·Adam J EnglerDennis E Discher
Jun 2, 2007·Cell·Shihuan KuangMichael A Rudnicki
Sep 20, 2008·Stem Cells·Darko BosnakovskiMichael Kyba
Sep 23, 2008·Nature·Alessandra SaccoHelen M Blau
Nov 27, 2009·Nature·Matthias P LutolfHelen M Blau
Dec 22, 2009·Integrative Biology : Quantitative Biosciences From Nano to Macro·Matthias P LutolfHelen M Blau

❮ Previous
Next ❯

Citations

May 31, 2012·Histochemistry and Cell Biology·Faisal Yusuf, Beate Brand-Saberi
Mar 23, 2011·Annals of Biomedical Engineering·Ngan F Huang, Song Li
Nov 22, 2011·Annals of Biomedical Engineering·Faramarz EdalatAli Khademhosseini
Jul 23, 2013·Biomaterials·Soraya Rasi GhaemiNicolas H Voelcker
Jun 25, 2013·Differentiation; Research in Biological Diversity·Jae-Won ShinDennis E Discher
May 28, 2013·Journal of Biotechnology·Binata Joddar, Yoshihiro Ito
Jul 31, 2013·Trends in Molecular Medicine·Alessandro FanzaniGuglielmo Sorci
Nov 9, 2012·Analytical Chemistry·Takeaki OzawaSung Bae Kim
May 25, 2012·Chemical Reviews·Akon HiguchiAkihiro Umezawa
Feb 9, 2013·Chemical Reviews·Akon HiguchiAkihiro Umezawa
Jan 16, 2013·Molecular Therapy : the Journal of the American Society of Gene Therapy·Naosumi SekiyaJohnny Huard
Dec 13, 2012·Nature Communications·Jason A Burdick, William L Murphy
Jun 8, 2013·Nature Communications·Anna UrciuoloPaolo Bonaldo
Jul 4, 2012·Nature Materials·Jing LiuBo Huang
Oct 11, 2011·Nature Methods·Samy GobaaMatthias P Lutolf
Sep 3, 2011·Nature Reviews. Molecular Cell Biology·Leo D Wang, Amy J Wagers
Dec 22, 2011·Nature Reviews. Molecular Cell Biology·Yu Xin Wang, Michael A Rudnicki
Aug 17, 2012·Nature Reviews. Molecular Cell Biology·Georg HalderStefano Piccolo
Jul 11, 2013·Nature Reviews. Molecular Cell Biology·Fiona M Watt, Wilhelm T S Huck
Jun 15, 2011·Integrative Biology : Quantitative Biosciences From Nano to Macro·Perla Ayala, Tejal A Desai
Feb 14, 2012·Integrative Biology : Quantitative Biosciences From Nano to Macro·Penney M GilbertHelen M Blau
Feb 22, 2012·Integrative Biology : Quantitative Biosciences From Nano to Macro·Florian RehfeldtDennis E Discher
Sep 1, 2012·Integrative Biology : Quantitative Biosciences From Nano to Macro·Raymond H W LamJianping Fu
Jan 23, 2013·Integrative Biology : Quantitative Biosciences From Nano to Macro·Yubing Sun, Jianping Fu
May 7, 2013·Lab on a Chip·Anja van de Stolpe, Jaap den Toonder
Nov 13, 2010·Proceedings of the National Academy of Sciences of the United States of America·Chong Yon ParkDeepak Srivastava
Aug 9, 2012·Proceedings of the National Academy of Sciences of the United States of America·Brendon M BakerRobert L Mauck
Apr 12, 2013·Proceedings of the National Academy of Sciences of the United States of America·Joseph E MarturanoCatherine K Kuo
Jul 19, 2013·Journal of Biomaterials Science. Polymer Edition·Xiufeng HuWenguang Liu
Oct 23, 2013·Computer Methods in Biomechanics and Biomedical Engineering·Bart SmeetsHans Van Oosterwyck
Apr 2, 2011·Rejuvenation Research·Anthony OttoKetan Patel
Jun 28, 2011·Tissue Engineering. Part a·Raymond L PageTanja Dominko
May 23, 2012·Tissue Engineering. Part a·Claire MongeCatherine Picart
Dec 21, 2012·Tissue Engineering. Part B, Reviews·Matthew B Fisher, Robert L Mauck
May 18, 2013·Tissue Engineering. Part B, Reviews·Jonathan Mark FishmanPaolo De Coppi

❮ Previous
Next ❯

Related Concepts

Related Feeds

Adult Stem Cells

Adult stem cells reside in unique niches that provide vital cues for their survival, self-renewal, and differentiation. They hold great promise for use in tissue repair and regeneration as a novel therapeutic strategies. Here is the latest research.