Substrate hydrophobicity and cell composition influence the extent of rate limitation and masking of isotope fractionation during microbial reductive dehalogenation of chlorinated ethenes

Environmental Science & Technology
Julian RenpenningIvonne Nijenhuis

Abstract

This study investigated the effect of intracellular microscale mass transfer on microbial carbon isotope fractionation of tetrachloroethene (PCE) and trichloroethene (TCE). Significantly stronger isotope fractionation was observed for crude extracts vs intact cells of Sulfurospirillum multivorans, Geobacter lovleyi, Desulfuromonas michiganensis, Desulfitobacterium hafniense strain PCE-S, and Dehalobacter restrictus. Furthermore, carbon stable isotope fractionation was stronger for microorganisms with a Gram-positive cell envelope compared to those with a Gram-negative cell envelope. Significant differences were observed between model organisms in cellular sorption capacity for PCE (S. multivorans-K(d-PCE) = 0.42-0.51 L g(-1); D. hafniense-K(d-PCE) = 0.13 L g(-1)), as well as in envelope hydrophobicity (S. multivorans 33.0° to 72.2°; D. hafniense 59.1° to 60.8°) when previously cultivated with fumarate or PCE as electron acceptor, but not for TCE. Cell envelope properties and the tetrachloroethene reductive dehalogenase (PceA-RDase) localization did not result in significant effects on observed isotope fractionation of TCE. For PCE, however, systematic masking of isotope effects as a result of microscale mass transfer limitation...Continue Reading

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Citations

Jan 17, 2016·Current Opinion in Biotechnology·Ivonne Nijenhuis, Kevin Kuntze
Jul 22, 2015·Environmental Science & Technology·Marco RattiThomas B Hofstetter
Jun 29, 2016·Current Opinion in Biotechnology·Ivonne Nijenhuis, Hans H Richnow
Aug 17, 2016·Environmental Science & Technology·Kevin KuntzeFaina Gelman
Sep 16, 2017·Environmental Science and Pollution Research International·Simon LeitnerAndrea Watzinger
Aug 15, 2017·Rapid Communications in Mass Spectrometry : RCM·Simon LeitnerAndrea Watzinger
Oct 22, 2018·Water Research·Guojun ChenLiping Qin
Dec 6, 2018·Environmental Science & Technology·Benno N EhrlMartin Elsner

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