Substrate kinetics and substrate effects on the quaternary structure of barley UDP-glucose pyrophosphorylase

Phytochemistry
Daniel DeckerLeszek A Kleczkowski

Abstract

UDP-Glc pyrophosphorylase (UGPase) is an essential enzyme responsible for production of UDP-Glc, which is used in hundreds of glycosylation reactions involving addition of Glc to a variety of compounds. In this study, barley UGPase was characterized with respect to effects of its substrates on activity and quaternary structure of the protein. Its K(m) values with Glc-1-P and UTP were 0.33 and 0.25 mM, respectively. Besides using Glc-1-P as a substrate, the enzyme had also considerable activity with Gal-1-P; however, the K(m) for Gal-1-P was very high (>10 mM), rendering this reaction unlikely under physiological conditions. UGPase had a relatively broad pH optimum of 6.5-8.5, regardless of the direction of reaction. The enzyme equilibrium constant was 0.4, suggesting slight preference for the Glc-1-P synthesis direction of the reaction. The quaternary structure of the enzyme, studied by Gas-phase Electrophoretic Mobility Macromolecule Analysis (GEMMA), was affected by addition of either single or both substrates in either direction of the reaction, resulting in a shift from UGPase dimers toward monomers, the active form of the enzyme. The substrate-induced changes in quaternary structure of the enzyme may have a regulatory role...Continue Reading

References

May 15, 1994·Journal of Biotechnology·L Elling, M R Kula
Dec 22, 1999·Biochimica Et Biophysica Acta·L A Kleczkowski
May 9, 2000·Biochemical and Biophysical Research Communications·K Lai, L J Elsas
Mar 17, 2004·Plant Physiology·Leszek A KleczkowskiHenrik Johansson
Aug 25, 2004·The Journal of Biological Chemistry·Toshihisa KotakeYoichi Tsumuraya
Jun 24, 2005·Proceedings of the National Academy of Sciences of the United States of America·Nicolas E BuchlerTerence Hwa
Nov 18, 2005·Phytochemistry·Leszek A KleczkowskiMalgorzata Wilczynska
Nov 23, 2005·Bioinformatics·Konstantin ArnoldTorsten Schwede
Jan 18, 2006·Plant Physiology·Jane Geisler-LeeEwa J Mellerowicz
Aug 1, 1986·Plant Physiology·L A Kleczkowski, D D Randall
Jun 8, 2006·Plant Physiology and Biochemistry : PPB·L A LittererD A Somers
Oct 3, 2006·Journal of Molecular Biology·Annette RoebenAndreas Bracher
Feb 17, 2007·The Journal of Biological Chemistry·Thomas SteinerUwe Jacob
Jan 10, 2008·Journal of Experimental Botany·Heather D ColemanShawn D Mansfield
Apr 16, 2009·Plant & Cell Physiology·Meng MengLeszek A Kleczkowski
Aug 27, 2009·BioEssays : News and Reviews in Molecular, Cellular and Developmental Biology·Abir U Igamberdiev, Leszek A Kleczkowski
Nov 13, 2009·The Journal of Biological Chemistry·Sebastian DamerowFrançoise H Routier
Mar 30, 2011·Plant Physiology·Leszek A KleczkowskiMalgorzata Wilczynska
Jul 14, 2011·The Biochemical Journal·Abir U Igamberdiev, Leszek A Kleczkowski

❮ Previous
Next ❯

Citations

Dec 21, 2012·Glycobiology·Jana FühringRita Gerardy-Schahn
Nov 16, 2013·Journal of Bioscience and Bioengineering·Yusuke KawanoMasaki Ihara
Mar 9, 2017·The Plant Journal : for Cell and Molecular Biology·Daniel DeckerLeszek A Kleczkowski
May 8, 2013·International Journal of Molecular Sciences·Matías D Asención DiezAlberto A Iglesias
Jan 23, 2020·The Biochemical Journal·Leszek A Kleczkowski, Abir U Igamberdiev
Oct 17, 2014·The Journal of Biological Chemistry·Jose Sergio M SoaresMarcelo Menossi
Oct 4, 2017·Frontiers in Plant Science·Daniel Decker, Leszek A Kleczkowski

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.