PMID: 12787242Jun 6, 2003Paper

Substrate preference of stress-activated phospholipase D in Chlamydomonas and its contribution to PA formation

The Plant Journal : for Cell and Molecular Biology
S A AriszT Munnik

Abstract

In response to various environmental stress conditions, plants rapidly form the intracellular lipid second messenger phosphatidic acid (PA). It can be generated by two independent signalling pathways via phospholipase D (PLD) and via phospholipase C (PLC) in combination with diacylglycerol kinase (DGK). In the green alga Chlamydomonas, the phospholipid substrates for these pathways are characterized by specific fatty acid compositions. This allowed us to establish: (i) PLD's in vivo substrate preference; and (ii) PLD's contribution to PA formation during stress signalling. Accordingly, G-protein activation (1 micro m mastoparan), hyperosmotic stress (150 mm NaCl) and membrane depolarization (50 mm KCl) were used to stimulate PLD, as monitored by the accumulation in 5 min of its unique transphosphatidylation product phosphatidylbutanol (PBut). In each case, PBut's fatty acid composition specifically matched that of phosphatidylethanolamine (PE), identifying this lipid as PLD's favoured substrate. This conclusion was substantiated by analysing the molecular species by electrospray ionization-mass spectrometry (ESI-MS/MS), which revealed that PE and NaCl-induced PBut share a unique (18 : 1)2-structure. The fatty acid composition o...Continue Reading

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Citations

Feb 14, 2009·Journal of Experimental Botany·Matthias Josef AffenzellerUrsula Lütz-Meindl
Apr 17, 2009·Plant & Cell Physiology·Essam DarwishTeun Munnik
Mar 18, 2016·Journal of Phycology·María Verónica BeligniArjen Ten Have
Apr 28, 2009·Biochimica Et Biophysica Acta·Steven A AriszTeun Munnik
Apr 6, 2005·The Plant Journal : for Cell and Molecular Biology·Christine ZalejskiEmmanuelle Jeannette
Jun 18, 2004·The Plant Journal : for Cell and Molecular Biology·Camiel F de JongTeun Munnik
Jul 27, 2004·The Plant Journal : for Cell and Molecular Biology·Christa TesterinkTeun Munnik
Jun 9, 2009·The Plant Journal : for Cell and Molecular Biology·Michael MishkindTeun Munnik
Jan 1, 2014·Environmental Microbiology·Marie-Mathilde PerrineauDebashish Bhattacharya
May 12, 2004·Current Opinion in Plant Biology·Xuemin Wang
Aug 1, 2006·Current Opinion in Plant Biology·Bastiaan O Bargmann, Teun Munnik
Feb 24, 2006·Progress in Lipid Research·Irina A Guschina, John L Harwood
Jul 19, 2005·Trends in Plant Science·Christa Testerink, Teun Munnik
Sep 25, 2003·Annual Review of Plant Biology·Harold J G Meijer, Teun Munnik
May 12, 2018·International Journal of Molecular Sciences·Xiao-Long HuWei-Qi Li

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