Substrate recognition and cleavage-site selection by a single-subunit protein-only RNase P

Nucleic Acids Research
Nadia BrillanteRoland K Hartmann

Abstract

RNase P is the enzyme that removes 5' extensions from tRNA precursors. With its diversity of enzyme forms-either protein- or RNA-based, ranging from single polypeptides to multi-subunit ribonucleoproteins-the RNase P enzyme family represents a unique model system to compare the evolution of enzymatic mechanisms. Here we present a comprehensive study of substrate recognition and cleavage-site selection by the nuclear single-subunit proteinaceous RNase P PRORP3 from Arabidopsis thaliana. Compared to bacterial RNase P, the best-characterized RNA-based enzyme form, PRORP3 requires a larger part of intact tRNA structure, but little to no determinants at the cleavage site or interactions with the 5' or 3' extensions of the tRNA. The cleavage site depends on the combined dimensions of acceptor stem and T domain, but also requires the leader to be single-stranded. Overall, the single-subunit PRORP appears mechanistically more similar to the complex nuclear ribonucleoprotein enzymes than to the simpler bacterial RNase P. Mechanistic similarity or dissimilarity among different forms of RNase P thus apparently do not necessarily reflect molecular composition or evolutionary relationship.

References

Aug 17, 1990·Science·A C Forster, S Altman
Oct 23, 1987·Science·W H McClainS Altman
Jul 14, 1989·Cell·G CarraraG P Tocchini-Valentini
Mar 28, 1995·Proceedings of the National Academy of Sciences of the United States of America·G CarraraG P Tocchini-Valentini
May 26, 1995·The Journal of Biological Chemistry·W RossmanithE Sbisà
Feb 21, 1998·Nucleic Acids Research·M SprinzlS Steinberg
Dec 24, 1997·Molecular Biology Reports·W RossmanithR M Karwan
Dec 23, 1998·Proceedings of the National Academy of Sciences of the United States of America·S NiranjanakumariC A Fierke
Jul 3, 1999·Journal of Molecular Biology·J M WarneckeR K Hartmann
Jun 3, 2004·Genome Research·Gavin E CrooksSteven E Brenner
Apr 28, 2006·Journal of Molecular Biology·Ema KikovskaLeif A Kirsebom
Feb 8, 2007·Proceedings of the National Academy of Sciences of the United States of America·Ema KikovskaLeif A Kirsebom
Aug 28, 2007·Journal of Molecular Biology·Mathias BrännvallLeif A Kirsebom
Sep 21, 2007·Biochimie·Dan LiRoland K Hartmann
Jan 23, 2008·Biological Chemistry·Michal MarszalkowskiRoland K Hartmann
Oct 30, 2008·Nucleic Acids Research·Frank JühlingJoern Pütz
Nov 26, 2009·Journal of Molecular Biology·Kristin S KoutmouCarol A Fierke
May 18, 2010·Nature Structural & Molecular Biology·Anthony GobertPhilippe Giegé
Jul 16, 2010·RNA·Olga Esakova, Andrey S Krasilnikov
Jul 28, 2010·Nucleic Acids Research·Antonio PlacidoLaurence Maréchal-Drouard
Nov 16, 2010·Nature·Nicholas J ReiterAlfonso Mondragón
Oct 1, 2011·Wiley Interdisciplinary Reviews. RNA·Richard GiegéCatherine Florentz
Oct 13, 2011·Molecular Systems Biology·Fabian SieversDesmond G Higgins
May 3, 2012·Genes & Development·Bernard GutmannPhilippe Giegé
Sep 15, 2012·Chembiochem : a European Journal of Chemical Biology·Liudmila V PavlovaRoland K Hartmann
Sep 20, 2012·Proceedings of the National Academy of Sciences of the United States of America·Michael J HowardMarkos Koutmos

❮ Previous
Next ❯

Citations

Oct 27, 2017·Proceedings of the National Academy of Sciences of the United States of America·Astrid I NickelRoland K Hartmann
Sep 9, 2018·Chembiochem : a European Journal of Chemical Biology·Seth E LyonVenkat Gopalan
Nov 19, 2019·Database : the Journal of Biological Databases and Curation·Alessandro La FerlitaAlfredo Pulvirenti
Jun 29, 2021·ELife·Rebecca FeyhFlorian Altegoer
Sep 8, 2021·Nature Structural & Molecular Biology·Arjun BhattaHauke S Hillen
Sep 11, 2021·Journal of Microbiological Methods·Sweetha GanapathyRoland K Hartmann

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.