PMID: 2484027Jul 15, 1988Paper

Substrate recognition by UDP-N-acetyl-alpha-D-galactosamine: polypeptide n-acetyl-alpha-D-galactosaminyltransferase. Effects of chain length and disulphide bonding of synthetic peptide substrates

Carbohydrate Research
R C HughesD G Smyth

Abstract

A synthetic peptide AcTPPP, based on a threonine-containing sequence present in bovine myelin basic protein, is a potent acceptor of glycosyl transfer from UDP-N-acetylgalactosamine catalyzed by extracts of baby hamster kidney (BHK) cells or rabbit lymph node tissue. In contrast, the disulphide-linked peptide (AcTCPPP)2, based on a glycosylated sequence present in the hinge region of rabbit immunoglobulin G, is not an acceptor and inhibits glycosylation of AcTPPP. Extension of the cystine-containing peptide at the N-terminus produced weak acceptors but strong acceptors resulted when the cystine residue was reduced to form monomeric peptides. The acceptor specificity of the N-acetylgalactosaminyl-transferase activity of BHK cells is very similar to that of rabbit lymph node tissue. The results indicated that tissues actively secreting immunoglobulin do not contain a transferase activity adapted specifically for glycosylation of sequences containing cystine residues, and suggested that addition of an N-acetylgalactosamine to a threonine residue in the hinge region of rabbit immunoglobulin takes place during biosynthesis prior to the formation of the inter-chain disulphide bridge of fully assembled immunoglobulins.

References

Jun 1, 1979·Proceedings of the National Academy of Sciences of the United States of America·G J Strous
Aug 1, 1976·Archives of Biochemistry and Biophysics·J P AubertM H Loucheux-Lefebvre
Oct 28, 1967·Nature·D S Smyth, S Utsumi
Nov 8, 1983·Biochimica Et Biophysica Acta·T F Cruz, M A Moscarello
Feb 1, 1984·European Journal of Biochemistry·D StojanovicR C Hughes
Jan 1, 1982·Annals of the New York Academy of Sciences·F W PutnamB Debuire

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Citations

Oct 31, 1991·Biochemical and Biophysical Research Communications·B O'ConnellN Ramasubbu
Jul 12, 2005·Bioorganic & Medicinal Chemistry·Howard C Hang, Carolyn R Bertozzi
Jan 1, 1993·Critical Reviews in Clinical Laboratory Sciences·I Brockhausen

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