Substrate recognition by unsaturated glucuronyl hydrolase from Bacillus sp. GL1

Biochemical and Biophysical Research Communications
Takafumi ItohK Murata

Abstract

Bacterial unsaturated glucuronyl hydrolases (UGLs) together with polysaccharide lyases are responsible for the complete depolymerization of mammalian extracellular matrix glycosaminoglycans. UGL acts on various oligosaccharides containing unsaturated glucuronic acid (DeltaGlcA) at the nonreducing terminus and releases DeltaGlcA through hydrolysis. In this study, we demonstrate the substrate recognition mechanism of the UGL of Bacillus sp. GL1 by determining the X-ray crystallographic structure of its substrate-enzyme complexes. The tetrasaccharide-enzyme complex demonstrated that at least four subsites are present in the active pocket. Although several amino acid residues are crucial for substrate binding, the enzyme strongly recognizes DeltaGlcA at subsite -1 through the formation of hydrogen bonds and stacking interactions, and prefers N-acetyl-d-galactosamine and glucose rather than N-acetyl-d-glucosamine as a residue accommodated in subsite +1, due to the steric hindrance.

References

Jan 1, 1978·Annual Review of Biochemistry·U Lindahl, M Höök
Jul 1, 1995·FEMS Microbiology Reviews·I W Sutherland
Jan 1, 1995·Critical Reviews in Biochemistry and Molecular Biology·S ErnstR Sasisekharan
May 23, 1996·Nature·R W HooftE E Abola
Apr 1, 1996·Applied and Environmental Microbiology·W HashimotoK Murata
Oct 3, 1998·Acta Crystallographica. Section D, Biological Crystallography·A T BrüngerG L Warren
Oct 6, 1998·Annual Review of Biochemistry·R V Iozzo
Aug 22, 2002·Journal of the American Chemical Society·Anu KoivulaT Alwyn Jones
Dec 12, 2002·The Journal of Biological Chemistry·Wataru HashimotoKousaku Murata
Jun 5, 2003·Acta Crystallographica. Section D, Biological Crystallography·Shigetarou MoriKousaku Murata
Feb 5, 2004·Archives of Biochemistry and Biophysics·Osamu MiyakeKousaku Murata
Nov 1, 1994·Acta Crystallographica. Section D, Biological Crystallography·E A Merritt, M E Murphy
Jan 1, 1997·Methods in Enzymology·Zbyszek Otwinowski, Wladek Minor

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Citations

Jul 26, 2008·Applied Microbiology and Biotechnology·Holy RavalasonIsabelle Herpoël-Gimbert
Aug 21, 2013·Accounts of Chemical Research·Seino A K Jongkees, Stephen G Withers
May 7, 2009·The Journal of Biological Chemistry·Yukie MaruyamaKousaku Murata
Feb 8, 2008·Bioscience, Biotechnology, and Biochemistry·Kousaku MurataWataru Hashimoto
Aug 8, 2015·Acta Crystallographica. Section F, Structural Biology Communications·Katherine L GermaneMargaret M Hurley
Apr 24, 2021·Chembiochem : a European Journal of Chemical Biology·Marcus BäumgenUwe T Bornscheuer

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