Substrate recognition drives the evolution of serine proteases.

The Journal of Biological Chemistry
T Rose, E Di Cera

Abstract

A method is introduced to identify amino acid residues that dictate the functional diversity acquired during evolution in a protein family. Using over 80 enzymes of the chymotrypsin family, we demonstrate that the general organization of the phylogenetic tree and its functional branch points are fully accounted for by a limited number of residues that cluster around the active site of the protein and define the contact region with the P1-P4 residues of substrate.

References

Jan 20, 1967·Science·W M Fitch, E Margoliash
Dec 5, 1993·Journal of Molecular Biology·A Sali, T L Blundell
Mar 29, 1996·Journal of Molecular Biology·O LichtargeF E Cohen
Sep 1, 1997·Nucleic Acids Research·S F AltschulD J Lipman
Sep 25, 1999·The Journal of Biological Chemistry·M M KremE Di Cera
Dec 11, 1999·Nucleic Acids Research·H M BermanP E Bourne
Dec 22, 1999·Molecular Biology and Evolution·X Gu

❮ Previous
Next ❯

Citations

Apr 6, 2006·Omics : a Journal of Integrative Biology·Andrey FomenkoVladimir Poroikov
Feb 24, 2006·Biochemistry. Biokhimii︠a︡·M E GasparianM P Kirpichnikov
Sep 4, 2008·Journal of Bioinformatics and Computational Biology·Kirill AlexandrovVladimir Poroikov
Nov 19, 2013·PLoS Computational Biology·Julian E FuchsKlaus R Liedl
Oct 26, 2005·Journal of Thrombosis and Haemostasis : JTH·M J PageE Di Cera
Feb 17, 2006·Blood Cells, Molecules & Diseases·Wolfram Bode
Apr 13, 2005·Biochemical and Biophysical Research Communications·Santad ChanprapaphGerd Katzenmeier
May 17, 2005·Anais Da Academia Brasileira De Ciências·Robson Q Monteiro
Nov 16, 2005·The Journal of Biological Chemistry·Patricia J AndersonJ Evan Sadler
Oct 9, 2002·The Journal of Biological Chemistry·Steven J OrcuttSriram Krishnaswamy
May 5, 2004·The Journal of Biological Chemistry·Sandra Waugh RugglesCharles S Craik
Apr 23, 2003·The Journal of Immunology : Official Journal of the American Association of Immunologists·Yuichi EndoTeizo Fujita
Jun 19, 2007·Molecular Biology and Evolution·Mara K N Lawniczak, David J Begun
Dec 25, 2004·The Biochemical Journal·Robson Q MonteiroIvo M B Francischetti
Aug 16, 2002·The Journal of Biological Chemistry·Maxwell M KremEnrico Di Cera
Jul 9, 2003·The Journal of Biological Chemistry·Maxwell M Krem, Enrico Di Cera
Nov 23, 2006·The Journal of Immunology : Official Journal of the American Association of Immunologists·Yao-Hsu YangPojen P Chen
Oct 1, 2020·Saudi Journal of Biological Sciences·Raha OrfaliRazan S Orfali

❮ Previous
Next ❯

Related Concepts

Related Feeds

ASBMB Publications

The American Society for Biochemistry and Molecular Biology (ASBMB) includes the Journal of Biological Chemistry, Molecular & Cellular Proteomics, and the Journal of Lipid Research. Discover the latest research from ASBMB here.