Substrate specificity and inhibition of brassinin hydrolases, detoxifying enzymes from the plant pathogens Leptosphaeria maculans and Alternaria brassicicola

The FEBS Journal
M Soledade C PedrasVijay K Sarma-Mamillapalle

Abstract

Blackleg (Leptosphaeria maculans and Leptosphaeria biglobosa) and black spot (Alternaria brassicicola) fungi are devastating plant pathogens known to detoxify the plant defence metabolite, brassinin. The significant roles of brassinin as a crucifer phytoalexin and as a biosynthetic precursor of several other plant defences make it important in plant fitness. Brassinin detoxifying enzymes produced by L. maculans and A. brassicicola catalyse the detoxification of brassinin by hydrolysis of its dithiocarbamate group to indolyl-3-methanamine. The purification and characterization of brassinin hydrolases produced by L. maculans (BHLmL2) and A. brassicicola (BHAb) were accomplished: native BHLmL2 was found to be a tetrameric protein with a molecular mass of 220 kDa, whereas native BHAb was found to be a dimeric protein of 120 kDa. Protein characterization using LC-MS/MS and sequence alignment analyses suggested that both enzymes belong to the family of amidases with the catalytic Ser/Ser/Lys triad. Furthermore, chemical modification of BHLmL2 and BHAb with selective reagents suggested that the amino acid serine was involved in the catalytic activity of both enzymes. The overall results indicated that BHs have new substrate specificit...Continue Reading

References

Jul 6, 1990·Biochimica Et Biophysica Acta·E VanderwinkelJ P ten Have
Jan 25, 2002·Acta Crystallographica. Section D, Biological Crystallography·Sebastian NeumannJörg Labahn
Aug 1, 2002·Biotechnology and Applied Biochemistry·G Rasul ChaudhrySheikh Riazuddin
Oct 9, 2002·Current Protein & Peptide Science·M Holmquist
Oct 3, 2003·Biochemical and Biophysical Research Communications·Jongchul YoonYoungsoo Kim
Feb 8, 2005·Phytochemistry·M Soledade C Pedras, Pearson W K Ahiahonu
Feb 16, 2007·Current Opinion in Plant Biology·M Eric SchranzThomas Mitchell-Olds
Mar 28, 2007·Analytica Chimica Acta·O H J Szolar
Apr 3, 2008·Chemical Record : an Official Publication of the Chemical Society of Japan ... [et Al.]·M Soledade C Pedras
Aug 30, 2008·Proceedings of the National Academy of Sciences of the United States of America·Mauro MileniRaymond C Stevens
Oct 1, 2008·Protein Science : a Publication of the Protein Society·Ozlem Doğan EkiciRoss E Dalbey
Oct 23, 2008·Biotechnology and Bioengineering·Sonja HeumannGeorg M Guebitz
Nov 6, 2008·Journal of Medicinal Chemistry·Mark Seierstad, J Guy Breitenbucher
Feb 27, 2009·Journal of Agricultural and Food Chemistry·M Soledade C PedrasVijay K Sarma-Mamillapalle

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Citations

Jul 25, 2012·Journal of Agricultural and Food Chemistry·M Soledade C Pedras, Vijay K Sarma-Mamillapalle
Jun 18, 2011·Natural Product Reports·M Soledade C PedrasErich Glawischnig
Jul 26, 2014·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Yangrae ChoJong Seog Ahn
Jul 2, 2014·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Alana Poloni, Jan Schirawski
Apr 27, 2010·Phytochemistry·M Soledade C Pedras, Estifanos E Yaya
Oct 12, 2016·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Diego QuirogaEricsson Coy-Barrera
Aug 16, 2013·Molecular Plant-microbe Interactions : MPMI·Akhil SrivastavaYangrae Cho
Feb 9, 2021·Frontiers in Plant Science·Guillaume Quang N'GuyenThomas Guillemette
May 18, 2021·Frontiers in Plant Science·Nathaniel M WestrickMehdi Kabbage

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