Substrate specificity for rat cytochrome P450 (CYP) isoforms: screening with cDNA-expressed systems of the rat

Biochemical Pharmacology
Kaoru KobayashiKan Chiba

Abstract

In this study, we performed a screening of the specificities of rat cytochrome P450 (CYP) isoforms for metabolic reactions known as the specific probes of human CYP isoforms, using 13 rat CYP isoforms expressed in baculovirus-infected insect cells or B-lymphoblastoid cells. Among the metabolic reactions studied, diclofenac 4-hydroxylation (DFH), dextromethorphan O-demethylation (DMOD) and midazolam 4-hydroxylation were specifically catalyzed by CYP2C6, CYP2D2 and CYP3A1/3A2, respectively. These results suggest that diclofenac 4-hydroxylation, dextromethorphan O-demethylation and midazolam 4-hydroxylation are useful as catalytic markers of CYP2C6, CYP2D2 and CYP3A1/3A2, respectively. On the other hand, phenacetin O-deethylation and 7-ethoxyresorufin O-deethylation were catalyzed both by CYP1A2 and by CYP2C6. Benzyloxyresorufin O-dealkylation and pentoxyresorufin O-dealkylation were also catalyzed by CYP1A2 in addition to CYP2B1. Bufuralol 1'-hydroxylation was extensively catalyzed by CYP2D2 but also by CYP2C6 and CYP2C11. p-Nitrophenol 2-hydroxylation and chlorzoxazone 6-hydroxylation were extensively catalyzed by CYP2E1 but also by CYP1A2 and CYP3A1. Therefore, it is necessary to conduct further study to clarify whether these a...Continue Reading

References

Feb 1, 1992·The Biochemical Journal·D J Waxman, L Azaroff
Nov 1, 1990·Chemical Research in Toxicology·R PeterC S Yang
Feb 1, 1987·Xenobiotica; the Fate of Foreign Compounds in Biological Systems·G C KahnA R Boobis
Apr 17, 1995·Biochemical and Biophysical Research Communications·H Kawashima, H W Strobel
Dec 1, 1994·Xenobiotica; the Fate of Foreign Compounds in Biological Systems·V Nedelcheva, I Gut
Aug 30, 1994·Biochemical Pharmacology·M D BurkeR T Mayer
Feb 22, 1994·Biochemistry·J A GoldsteinB I Ghanayem

❮ Previous
Next ❯

Citations

Oct 28, 2005·European Journal of Drug Metabolism and Pharmacokinetics·M DostalekI Linhart
Feb 5, 2010·Archives of Toxicology·Kang Uk YunSang Kyum Kim
Jul 1, 2011·Archives of Toxicology·Sang Yoon LeeSang Kyum Kim
Aug 9, 2013·Biochemical Pharmacology·Anna Sadakierska-ChudyWładysława A Daniel
Feb 6, 2004·European Journal of Pharmacology·Liane D PaulHans H Maurer
Sep 3, 2003·European Journal of Biochemistry·Annalise Di MarcoRalph Laufer
Oct 28, 2009·Toxicological Sciences : an Official Journal of the Society of Toxicology·Emilija MakajiDenis J Crankshaw
Nov 10, 2009·Pharmacogenetics and Genomics·Caroline F ThornRuss B Altman
Aug 10, 2002·Journal of Cellular and Molecular Medicine·R ZuberP Anzenbacher
Jan 11, 2013·Biological & Pharmaceutical Bulletin·Junichi IidaKiyomi Ito
Jan 9, 2014·Pharmacological Reports : PR·Jacek WójcikowskiWładysława Anna Daniel
May 28, 2013·Evidence-based Complementary and Alternative Medicine : ECAM·Xin TianHai-Ling Qiao
Nov 27, 2014·European Journal of Drug Metabolism and Pharmacokinetics·Gurbet CelikAlaattin Sen
Mar 15, 2003·Drug Metabolism Reviews·Shufeng ZhouJames W Paxton
Apr 19, 2008·Xenobiotica; the Fate of Foreign Compounds in Biological Systems·Y DengS-F Zhou
Feb 10, 2016·Frontiers in Physiology·Divya SinghGhanshyam Upadhyay
Mar 29, 2011·Pharmacological Reports : PR·Aiming LiuRenke Dai
Jul 4, 2012·Pharmacological Research : the Official Journal of the Italian Pharmacological Society·Marta KotWładysława A Daniel
Oct 13, 2009·European Journal of Pharmacology·Ewa BromekWładysława A Daniel
Aug 26, 2009·New Biotechnology·Shazia TanvirSylviane Pulvin
Apr 14, 2009·Nitric Oxide : Biology and Chemistry·Anderson Rodrigo Moraes de OliveiraPierina Sueli Bonato
Jan 3, 2009·Toxicology and Applied Pharmacology·L RichertS O Mueller
Dec 2, 2008·European Journal of Pharmaceutical Sciences : Official Journal of the European Federation for Pharmaceutical Sciences·Xiuhua RenGao Li
Mar 18, 2008·Biochemical Pharmacology·Leslie J DickmannNina Isoherranen
Dec 7, 2007·Chemico-biological Interactions·I RodeiroM J Gómez-Lechón
Jul 27, 2007·European Journal of Pharmacology·Phisit KhemawootKen-ichi Miyamoto
Jul 3, 2004·The Journal of Pharmacy and Pharmacology·Jill S WarringtonLisa L von Moltke
Sep 7, 2007·Journal of Veterinary Pharmacology and Therapeutics·S S ShahM Shimoda
Sep 7, 2007·Journal of Veterinary Pharmacology and Therapeutics·K A CortrightA L Craigmill
Nov 7, 2006·Journal of Veterinary Pharmacology and Therapeutics·K A Cortright, A L Craigmill
Aug 19, 2007·Basic & Clinical Pharmacology & Toxicology·Mette T Skaanild, Christian Friis
Jan 15, 2013·Journal of Biochemical and Molecular Toxicology·Okechukwu UkairoEdward L Lecluyse
Jun 8, 2010·Basic & Clinical Pharmacology & Toxicology·Fang YuanMin Huang

❮ Previous
Next ❯

Related Concepts

Related Feeds

Antiparasitics

Antiparasitics are medications which are indicated for the treatment of parasitic diseases. Discover the latest research on antiparasitics here.